Andrabi S M, Ahmad M M, Shahab M
Department of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Vet Hum Toxicol. 1998 Dec;40(6):321-5.
We determined the effect of chronic administration of furazolidone (Fz) on sexual maturation of male broiler breeder birds (Ross 308; Gallus domesticus). A total of 20 15-w-old birds were randomly assigned to receive 0, 150, 250 or 350 mg Fz/kg feed daily for 5-w. Blood samples were taken at weekly intervals. The birds were challenged with 500 IU human chorionic gonadotropin (hCG) i.v. at the age of 24 w before slaughtering. Concentration of testosterone in the plasma was measured by a specific radioimmunoassay. Testicular tissue was processed for morphometric studies. Testicular weights of the groups fed 250 or 350 mg Fz/kg feed/d were decreased (P < 0.05). Plasma testosterone levels were affected by age (P < 0.001) and dose of Fz (P < 0.001). Mean plasma testosterone levels during and after drug administration were reduced (P < 0.05) by all Fz doses. Human chorionic gonadotropin administration led to poor testosterone response (P < 0.05) in all Fz-dosed groups, but not the control group (P < 0.05). As compared to the control, Fz-dosing reduced the seminiferous tubule diameter (P < 0.05) at the 350 mg/kg feed dose, seminiferous epithelial height (P < 0.05) at the 250 and 350 mg/kg feed doses, and Leydig cell nuclear diameter (P < 0.05) at the 350 mg/kg feed dose. This data suggest that sexual maturation in male broiler breeder birds is adversely affected by chronic Fz-administration. Actions of Fz on sexual maturation probably involve a direct effect at the testicular level.
我们测定了长期给予呋喃唑酮（Fz）对雄性肉种鸡（罗斯308；家鸡）性成熟的影响。总共20只15周龄的鸡被随机分配，每天分别接受0、150、250或350毫克Fz/千克饲料，持续5周。每周采集血样。在24周龄屠宰前，对鸡静脉注射500国际单位人绒毛膜促性腺激素（hCG）进行刺激。通过特定的放射免疫测定法测量血浆中睾酮的浓度。对睾丸组织进行形态计量学研究。饲喂250或350毫克Fz/千克饲料/天的组睾丸重量降低（P<0.05）。血浆睾酮水平受年龄（P<0.001）和Fz剂量（P<0.001）影响。所有Fz剂量在给药期间及之后均使平均血浆睾酮水平降低（P<0.05）。在所有Fz给药组中，注射人绒毛膜促性腺激素导致睾酮反应不佳（P<0.05），但对照组无此情况（P<0.05）。与对照组相比，350毫克/千克饲料剂量的Fz给药使生精小管直径减小（P<0.05），250和350毫克/千克饲料剂量使生精上皮高度降低（P<0.05），350毫克/千克饲料剂量使睾丸间质细胞核直径减小（P<0.05）。这些数据表明，长期给予Fz对雄性肉种鸡的性成熟有不利影响。Fz对性成熟的作用可能涉及在睾丸水平的直接影响。
