Department of Microbiology and Immunology, The School of Medicine, University of Tampere, Finland.
Immun Ageing. 2011 Oct 11;8:9. doi: 10.1186/1742-4933-8-9.
Old age is associated with increased levels of circulating pro-inflammatory cytokines, a phenomenon termed inflamm-aging. Elevated levels of pro-inflammatory cytokines have been associated with several age-associated diseases and with a shortened lifespan. Indoleamine 2,3-dioxygenase (IDO) has immunomodulatory properties and its activity is elevated in inflammation, autoimmune disorders and malignancies. We have previously shown that IDO activity is increased in nonagenarians compared to young individuals and that high IDO activity is associated with mortality at old age.
In this study our aim was to assess whether this difference in IDO activity in the plasma was due to the differential expression of either the IDO1 or IDO2 gene in peripheral blood mononuclear cells. Our results show that IDO1 and IDO2 are not differently expressed in nonagenarians compared to controls and that the expression of IDO genes is not associated with the level of IDO activity in the plasma.
The level of IDO activity in the plasma is not regulated through the expression of IDO1 or IDO2 in the peripheral blood mononuclear cells.
衰老与循环中促炎细胞因子水平升高有关，这种现象被称为炎症衰老。促炎细胞因子水平升高与几种与年龄相关的疾病和寿命缩短有关。吲哚胺 2,3-双加氧酶 (IDO) 具有免疫调节特性，其活性在炎症、自身免疫性疾病和恶性肿瘤中升高。我们之前曾表明，与年轻人相比，非百岁老人的 IDO 活性增加，并且高 IDO 活性与老年时的死亡率相关。
在这项研究中，我们的目的是评估血浆中 IDO 活性的这种差异是否是由于外周血单核细胞中 IDO1 或 IDO2 基因的差异表达所致。我们的结果表明，与对照组相比，非百岁老人的 IDO1 和 IDO2 表达没有差异，IDO 基因的表达与血浆中 IDO 活性水平无关。
血浆中 IDO 活性的水平不是通过外周血单核细胞中 IDO1 或 IDO2 的表达来调节的。
