Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Chembiochem. 2011 Nov 25;12(17):2547-50. doi: 10.1002/cbic.201100476. Epub 2011 Oct 11.
A facile pretreatment process for SEM: The use of room temperature ionic liquids (RTILs) provides an interesting method for SEM of biological specimens. We used a novel and concise method of pretreatment, excluding fixation or Au sputtering steps. Fine and smooth-textured SEM images of a wide variety of biological specimens treated in this way were observed without artefacts.
一种简单的 SEM 预处理方法：使用室温离子液体（RTILs）为 SEM 观察生物样本提供了一种有趣的方法。我们使用了一种新颖而简洁的预处理方法，不包括固定或 Au 溅射步骤。用这种方法处理的各种生物样本都能观察到精细、光滑的 SEM 图像，没有伪影。
