Jäger S, Menger M D, Göhde W, Feifel G
Department of General Surgery, University of Saarland Medical School, Homburg/Saar, FRG.
Eur Surg Res. 1990;22(1):8-13. doi: 10.1159/000129076.
We present a new technique for specific ex situ fluorescent staining of islets of Langerhans with neutral red. Depending on the concentration of neutral red (1 microgram/ml), this technique allows the differentiation between exocrine and endocrine pancreatic tissue, according to differences in fluorescence intensity. Pancreatic islets can be stained not only in situ, but in particular ex situ after pancreas digestion. Since intravital staining of the pancreas in situ with neutral red is not applicable in human transplantation, the staining procedure ex situ may be a useful tool for islet purification by particle sorting, using a fluorescent-light-activated system; thus, a sufficient mass of purified islets for free transplantation may be isolated.
我们提出了一种用中性红对朗格汉斯胰岛进行特异性异位荧光染色的新技术。根据中性红的浓度（1微克/毫升），该技术可根据荧光强度的差异区分胰腺外分泌组织和内分泌组织。胰岛不仅可以原位染色，特别是在胰腺消化后还可以异位染色。由于中性红对胰腺进行原位活体染色不适用于人类移植，异位染色程序可能是一种有用的工具，可通过使用荧光光激活系统进行颗粒分选来纯化胰岛；因此，可以分离出足够数量的用于自由移植的纯化胰岛。
