Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, Sweden.
Biochem J. 2011 Nov 1;439(3):375-9. doi: 10.1042/BJ20110730.
Plant pyrophosphorylases that are capable of producing UDP-sugars, key precursors for glycosylation reactions, include UDP-glucose pyrophosphorylases (A- and B-type), UDP-sugar pyrophosphorylase and UDP-N-acetylglucosamine pyrophosphorylase. Although not sharing significant homology at the amino acid sequence level, the proteins share a common structural blueprint. Their structures are characterized by the presence of the Rossmann fold in the central (catalytic) domain linked to enzyme-specific N-terminal and C-terminal domains, which may play regulatory functions. Molecular mobility between these domains plays an important role in substrate binding and catalysis. Evolutionary relationships and the role of (de)oligomerization as a regulatory mechanism are discussed.
能够产生 UDP-糖的植物焦磷酸化酶，是糖基化反应的关键前体，包括 UDP-葡萄糖焦磷酸化酶（A 型和 B 型）、UDP-糖焦磷酸化酶和 UDP-N-乙酰葡萄糖胺焦磷酸化酶。尽管在氨基酸序列水平上没有显著的同源性，但这些蛋白质共享一个共同的结构蓝图。它们的结构特征是中央（催化）结构域中存在 Rossmann 折叠，与酶特异性的 N-末端和 C-末端结构域相连，这些结构域可能发挥调节功能。这些结构域之间的分子迁移在底物结合和催化中起着重要作用。讨论了进化关系和（解）寡聚化作为调节机制的作用。
