利用长双歧杆菌（BLUSP）中的一种混杂的 UDP-糖焦磷酸化酶高效一锅多酶合成 UDP-糖。
Efficient one-pot multienzyme synthesis of UDP-sugars using a promiscuous UDP-sugar pyrophosphorylase from Bifidobacterium longum (BLUSP).
机构信息
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
出版信息
Chem Commun (Camb). 2012 Mar 11;48(21):2728-30. doi: 10.1039/c2cc17577k. Epub 2012 Feb 3.
A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) with or without a monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, and their derivatives. Further chemical diversification of a UDP-mannose derivative resulted in the formation of UDP-N-acetylmannosamine.
从长双歧杆菌 ATCC55813 菌株中克隆出一种混杂的 UDP-糖焦磷酸化酶（BLUSP），并与多杀巴斯德氏菌无机焦磷酸酶（PmPpA）一起使用，无论是否有单糖 1-激酶，都能有效地用于 UDP-半乳糖、UDP-葡萄糖、UDP-甘露糖及其衍生物的一锅多酶合成。进一步对 UDP-甘露糖衍生物进行化学多样化，得到 UDP-N-乙酰甘露糖胺。
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