Cátedra de Inmunología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
Parasite Immunol. 2011 Nov;33(11):621-31. doi: 10.1111/j.1365-3024.2011.01326.x.
Helminth parasite infections are associated with predominant Th2-type cytokine responses, and parasite glycoconjugates have been recognized as partially responsible for such immune bias. It has been proved that Echinococcus granulosus evokes a Th2-type cytokine pattern characterized by a high production of IL-4, IL-5, IL-6 and IL-10, and no or mild IFN-γ levels in animal models and in patients with cystic echinococcosis, respectively. Here, we show that E4(+) (a glycoconjugate-enriched fraction from E. granulosus protoscolex) stimulated the secretion of a high concentration of IL-6, followed by IL-10 and TNF-α by normal peritoneal B cells. We determined that E4(+) bound to the surface of peritoneal B cells and induced their activation and, also, triggered the differentiation of peritoneal B cells into IgM-, IgG2b- and IgG3-secreting cells in a T-independent way. Interestingly, the IgM released by E4(+) -stimulated peritoneal B cells from normal mice recognized protoscolex antigens. Results showed that, after the encounter with antigens from E. granulosus protoscolex, peritoneal B cells are a source of Th2-type cytokines and polyclonal antibodies, some of which recognize parasite antigens, suggesting that peritoneal B cells can condition the outcome of the infection.
寄生虫感染与 Th2 型细胞因子反应有关，寄生虫糖缀合物被认为是导致这种免疫偏向的部分原因。已经证明，细粒棘球绦虫会引起 Th2 型细胞因子模式，其特征是在动物模型和囊性包虫病患者中分别产生大量的 IL-4、IL-5、IL-6 和 IL-10，而 IFN-γ 水平较低或无。在这里，我们显示 E4(+)（从细粒棘球绦虫原头节中富含糖缀合物的部分）刺激正常腹膜 B 细胞分泌高浓度的 IL-6，随后是 IL-10 和 TNF-α。我们确定 E4(+) 与腹膜 B 细胞表面结合，诱导其激活，并以 T 细胞非依赖性方式触发腹膜 B 细胞分化为 IgM-、IgG2b- 和 IgG3 分泌细胞。有趣的是，E4(+)刺激的正常小鼠腹膜 B 细胞释放的 IgM 可识别原头节抗原。结果表明，在与细粒棘球绦虫原头节抗原相遇后，腹膜 B 细胞是 Th2 型细胞因子和多克隆抗体的来源，其中一些抗体可识别寄生虫抗原，这表明腹膜 B 细胞可以影响感染的结果。
