Pan Wei, Hao Wen-Ting, Shen Yu-Juan, Li Xiang-Yang, Wang Yan-Juan, Sun Fen-Fen, Yin Jian-Hai, Zhang Jing, Tang Ren-Xian, Cao Jian-Ping, Zheng Kui-Yang
Jiangsu Key Laboratory of Immunity and Metabolism; Department of Pathogenic Biology and Immunology, Laboratory of Infection and Immunity, Xuzhou Medical University, Xuzhou, Jiangsu Province, China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, China.
Parasit Vectors. 2017 Jul 21;10(1):348. doi: 10.1186/s13071-017-2263-9.
Excretory-secretory products (ESPs) released by helminths are well-known to regulate T cell responses in the host. However, their direct influence in the differentiation of naïve T cells, and especially B cells, remains largely unknown. This study investigated the effects of Echinococcus granulosus protoscoleces ESPs (EgPSC-ESPs) on the differentiation of IL-10-producing B cells (B10), IL-17A-producing B cells (B17) and Th17 cells.
BALB/c mice injected with EgPSC were used to evaluate the in vivo profiles of B10, B17 and Th17 cells. In vitro purified CD19 B and naïve CD4 T cells were cultured in the presence of native, heat-inactivated or periodate-treated EgPSC-ESPs, and the differentiation of these cell subsets were compared.
In contrast to the control group, infected mice showed higher frequencies of B10, B17 and Th17 cells, and higher levels of IL-10 and IL-17A in the sera. Interestingly, B17 cells were first identified to express CD19CD1d. In vitro, B cells cultured with native ESPs exhibited a higher percentage of B10 cells but lower percentage of B17 and Th17 cells compared to the PBS group. Moreover, the relative expression of IL-10 and IL-17A mRNA were consistent with the altered frequencies. However, ESPs subjected to heat-inactivation or periodate treatment exhibited an inverse effect on the induction of these cell subsets.
Our findings indicate that ESPs released by EgPSC can directly regulate the differentiation of B10, B17 and Th17 cells, which appear to be heat-labile and carbohydrate-dependent.
众所周知，蠕虫释放的排泄分泌产物（ESPs）可调节宿主体内的T细胞反应。然而，它们对初始T细胞，尤其是B细胞分化的直接影响仍 largely未知。本研究调查了细粒棘球绦虫原头节ESPs（EgPSC-ESPs）对产生白细胞介素-10的B细胞（B10）、产生白细胞介素-17A的B细胞（B17）和辅助性T细胞17（Th17）分化的影响。
注射了EgPSC的BALB/c小鼠用于评估B10、B17和Th17细胞的体内分布情况。体外纯化的CD19+B细胞和初始CD4+T细胞在天然、热灭活或经高碘酸盐处理的EgPSC-ESPs存在的情况下进行培养，并比较这些细胞亚群的分化情况。
与对照组相比，感染小鼠体内B10、B17和Th17细胞的频率更高，血清中白细胞介素-10和白细胞介素-17A的水平也更高。有趣的是，首次发现B17细胞表达CD19+CD1d。在体外，与PBS组相比，用天然ESPs培养的B细胞中B10细胞的百分比更高，但B17和Th17细胞的百分比更低。此外，白细胞介素-10和白细胞介素-17A mRNA的相对表达与细胞频率的变化一致。然而，经过热灭活或高碘酸盐处理的ESPs对这些细胞亚群的诱导作用则相反。
我们的研究结果表明，EgPSC释放的ESPs可直接调节B10、B17和Th17细胞的分化，这些调节作用似乎对热不稳定且依赖碳水化合物。
