Meng Fan-Mei, Zhang Chao-Hui, Ai Yun-Can
Sun Yat-sen University, Guangzhou, China.
Yi Chuan. 2011 Oct;33(10):1113-20. doi: 10.3724/sp.j.1005.2011.01113.
The phage display technology (PDT) was unique in genetic engineering and recombinant expression. The phage display systems (PDS) were platforms (kits) composed of genetic modified phages, helper phages, and host bacteria. This review concisely summarized the development of four types of PDS, based on M13, λ, T4, and T7 phages, in terms of phage molecular genetics and genetic (gene or genome) engineering. We addressed on the key components and their genetic (genomic) engineering for modifications, the technical features of different anchors, and the development progress and selection reference of those different kits.
噬菌体展示技术(PDT)在基因工程和重组表达方面独具特色。噬菌体展示系统(PDS)是由基因改造的噬菌体、辅助噬菌体和宿主细菌组成的平台(试剂盒)。本综述从噬菌体分子遗传学和遗传(基因或基因组)工程的角度,简要总结了基于M13、λ、T4和T7噬菌体的四种类型PDS的发展情况。我们探讨了用于修饰的关键组件及其遗传(基因组)工程、不同锚定物的技术特点,以及这些不同试剂盒的发展进展和选择参考。