Williams Jeannette N, Christodoulides Myron, Heckels John E
Division of Infection, Inflammation, and Immunity, Sir Henry Wellcome Laboratories, University of Southampton Medical School, Southampton, UK.
Methods Mol Biol. 2012;799:343-60. doi: 10.1007/978-1-61779-346-2_20.
The availability of Neisseria genome sequences together with improvements in proteomic technologies provide the opportunity to study at high resolution the immune response to Neisseria meningitidis. In this chapter, we describe a protocol that combines two-dimensional (2D) SDS-PAGE of meningococcal outer membranes with western blotting of human antisera to identify proteins associated with the development of protective antibody responses. This methodology can identify putative vaccine candidates for incorporation in a multi-component serogroup B meningococcal vaccine.
脑膜炎奈瑟菌基因组序列的可得性以及蛋白质组学技术的改进，为高分辨率研究针对脑膜炎奈瑟菌的免疫反应提供了机会。在本章中，我们描述了一种方案，该方案将脑膜炎球菌外膜的二维（2D）SDS-PAGE与人类抗血清的蛋白质印迹相结合，以鉴定与保护性抗体反应发展相关的蛋白质。这种方法可以识别可纳入多组分B群脑膜炎球菌疫苗的潜在疫苗候选物。
