制备规模等电聚焦通过在具有正交初级和次级 pH 梯度的分区系统中进行递归电泳。第 1 部分——构建和标准操作。
Preparative-scale isoelectric trapping by recursive electrophoresis in a compartmentalized system having orthogonal primary and secondary pH gradients. Part 1--construction and standard operation.
机构信息
Department of Chemistry, Texas A&M University, College Station, TX, USA.
出版信息
Electrophoresis. 2011 Oct;32(20):2797-804. doi: 10.1002/elps.201100119. Epub 2011 Oct 13.
The performance of the current preparative-scale isoelectric trapping systems is limited by the serial arrangement of the separation compartments. A new system has been developed that achieves trapping by recursive electrophoresis in a compartmentalized system (T-RECS). T-RECS features (i) parallel-connected elementary separation heads with independent electrode compartments, feed compartments, and harvest compartments, (ii) orthogonal primary pH gradients and secondary pH gradients, (iii) directionally controlled convective analyte transport along the primary (resolving) pH gradients, and (iv) electrophoretic analyte transport along the secondary (harvesting) pH gradients. The operation of T-RECS has been quantitatively characterized via separation of small molecule ampholytes.
当前的制备规模等电聚焦系统的性能受到分离隔室串联排列的限制。已经开发出一种新的系统，该系统通过在分区系统中进行递归电泳实现捕获（T-RECS）。T-RECS 的特点是：（i）具有独立电极隔室、进样隔室和收获隔室的并联基本分离头；（ii）正交的初级 pH 梯度和次级 pH 梯度；（iii）沿初级（解析）pH 梯度定向控制的对流分析物传输；（iv）沿次级（收获）pH 梯度进行电泳分析物传输。通过分离小分子两性电解质对 T-RECS 的操作进行了定量表征。
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