Department of Chemistry, Texas A&M University, College Station, TX, USA.
Electrophoresis. 2011 Oct;32(20):2805-8. doi: 10.1002/elps.201100120. Epub 2011 Oct 13.
A parallel multicompartmental electrolyzer recently developed for preparative-scale isoelectric trapping separations, trapping by recursive electrophoresis in a compartmentalized system, was set up to operate as a cascade of binary separations to produce at least one pure target ampholyte (or more, with additional separation heads) without other ampholytes ever entering (even transiently) the harvest stream. This mode of operation avoids the need for exhaustive electrophoresis and the accompanying long separation times brought about by the exponentially decreasing concentrations over the course of batch separations. Continuous operation can be achieved in the cascade mode by continuously feeding the sample into the first separation head configured with three flow-through compartments and continuously harvesting one (or more) target components in additional separation heads configured with two flow-through compartments, attached to the respective branching points.
最近开发的一种用于制备规模等电聚焦分离的平行多隔室电解槽，采用隔室递归电泳进行聚焦，可作为级联二元分离运行，可生产至少一种纯目标两性电解质（或更多，使用附加分离头），而其他两性电解质从未进入（甚至瞬时）收获流。这种操作模式避免了需要进行彻底的电泳以及由于批次分离过程中浓度呈指数下降而带来的长分离时间。在级联模式下，通过连续将样品进料到配置有三个流通隔室的第一个分离头，并连续收获在配置有两个流通隔室的附加分离头中的一个（或更多）目标成分，可以实现连续操作，这些分离头连接到相应的分支点。
