Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, 5019 Monastir, Tunisia.
Int J Toxicol. 2012 Jan-Feb;31(1):78-85. doi: 10.1177/1091581811423981. Epub 2011 Oct 12.
Dimethoate (DM) is an organophosphate insecticide with numerous uses on field and agricultural crops and ornamentals. Data concerning DM-acute genotoxicity are controversial and knowledge on its delayed effect is limited. For this reason, we aimed to further explore DM genotoxicity resulting from subchronic intoxication of experimental mice. Thus, DM was administered to mice at doses ranging from 1 to 30 mg/kg body weight for a period of 30 consecutive days. There was a significant increase (P < .05) in the frequency of micronucleated bone marrow cells following DM administration. Furthermore, the chromosome aberration assay revealed a significant increase in the percentage of chromosome abnormalities in a dose-dependent manner. Dimethoate was also found to induce significant DNA damage in mouse bone marrow cells as assessed by the comet assay. Altogether, our results showed that, after a subchronic exposure, DM was a genotoxic compound in experimental mice.
乐果（DM）是一种有机磷杀虫剂，在田间和农作物以及观赏作物上有多种用途。有关 DM 急性遗传毒性的数据存在争议，对其延迟效应的了解有限。因此，我们旨在进一步探索实验小鼠亚慢性中毒导致的 DM 遗传毒性。因此，DM 以 1 至 30 毫克/千克体重的剂量连续 30 天给予小鼠。DM 给药后，骨髓细胞微核的频率显着增加（P <.05）。此外，染色体畸变试验显示，染色体异常的百分比呈剂量依赖性增加。彗星试验还表明，DM 可诱导小鼠骨髓细胞发生显著的 DNA 损伤。总之，我们的研究结果表明，经过亚慢性暴露后，DM 是实验小鼠的一种遗传毒性化合物。
