Sarkar Saurabh, Roy Arnab, Roy Sumedha
Toxicology Research Unit, Cytogenetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, 713104, India.
Invert Neurosci. 2018 Apr 26;18(2):6. doi: 10.1007/s10158-018-0210-x.
Flubendiamide is widely used in agricultural fields to exterminate a broad spectrum of pests (lepidopteran insects) by disrupting their muscle function. The main objective of this study was to find the effects of flubendiamide on a non-target organism, Drosophila melanogaster (dipteran insect). In the present study, different sub-lethal concentrations of Flubendiamide caused a significant (P < 0.05) decrease in acetylcholinesterase activity and increase in cytochrome P450 activity in adult D. melanogaster. Phototaxis and climbing behaviours were found to significantly (P < 0.05) alter in exposed flies. The observed alteration in phototaxis and climbing behaviours were not restricted to P generation, but were found to be transmitted to subsequent generations (F and F generation) that had never been directly exposed to the test chemical during their life time. It is only their predecessors (P generation) who have been affronted with different concentrations of Flubendiamide. Humans and Drosophilids share almost 60% genomic similarity and 75% disease gene resemblance. Moreover, most of the circuits governing the behaviours studied involve the inhibition and excitation of neurotransmitters, which are conserved in humans and flies. Thus, the present findings suggest that chronic flubendiamide exposure might induce alteration in neurotransmission leading to discrepancy in the behavioural responses (vision and flight) in other beneficial insects and insect-dependent organisms.
氟苯虫酰胺被广泛应用于农业领域，通过破坏害虫（鳞翅目昆虫）的肌肉功能来消灭多种害虫。本研究的主要目的是探究氟苯虫酰胺对非靶标生物黑腹果蝇（双翅目昆虫）的影响。在本研究中，不同亚致死浓度的氟苯虫酰胺导致成年黑腹果蝇的乙酰胆碱酯酶活性显著降低（P < 0.05），细胞色素P450活性升高。暴露组果蝇的趋光性和攀爬行为发生了显著改变（P < 0.05）。观察到的趋光性和攀爬行为的改变不仅局限于P代，还发现会传递给在其生命周期中从未直接接触过受试化学物质的后代（F1和F2代）。只有它们的前代（P代）接触过不同浓度的氟苯虫酰胺。人类和果蝇的基因组相似度近60%，疾病基因相似度达75%。此外，大多数控制所研究行为的神经回路都涉及神经递质的抑制和兴奋，这在人类和果蝇中是保守的。因此，本研究结果表明，长期接触氟苯虫酰胺可能会导致神经传递改变，从而使其他有益昆虫和依赖昆虫的生物在行为反应（视觉和飞行）上出现差异。
