盘基网柄菌中一种68-kD蛋白质的快速翻译后豆蔻酰化。
A rapid posttranslational myristylation of a 68-kD protein in D. discoideum.
作者信息
da Silva A M, Klein C
机构信息
E. A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, Missouri 63104.
出版信息
J Cell Biol. 1990 Aug;111(2):401-7. doi: 10.1083/jcb.111.2.401.
Abstract
Cells incubated with [3H]myristate were shown to rapidly and specifically acylate a 68-kD protein, p68, in a developmentally-regulated manner. The fatty acid incorporated into p68 was identified as myristate, and is linked to the protein via an amide bond, apparently to an NH2-terminal glycine. The acylation of p68 in D. discoideum displays some unusual properties. Unexpectedly, myristylation of p68 is a posttranslational event and occurs in the presence of inhibitors of protein synthesis. Another unusual finding was that although p68 is a stable protein, the acyl moiety is removed with a half time of approximately 15 min.
摘要
用[3H]肉豆蔻酸盐孵育的细胞显示，能以发育调控的方式快速且特异性地将一种68-kD蛋白（p68）酰化。掺入p68的脂肪酸被鉴定为肉豆蔻酸盐，并且通过酰胺键与该蛋白相连，显然是与一个NH2端甘氨酸相连。盘基网柄菌中p68的酰化表现出一些不寻常的特性。出乎意料的是，p68的肉豆蔻酰化是一个翻译后事件，并且在存在蛋白质合成抑制剂的情况下发生。另一个不寻常的发现是，尽管p68是一种稳定的蛋白，但酰基部分以大约15分钟的半衰期被去除。
相似文献
1
A rapid posttranslational myristylation of a 68-kD protein in D. discoideum.盘基网柄菌中一种68-kD蛋白质的快速翻译后豆蔻酰化。
J Cell Biol. 1990 Aug;111(2):401-7. doi: 10.1083/jcb.111.2.401.
2
Specificity of fatty acid acylation of cellular proteins.细胞蛋白质的脂肪酸酰化特异性
J Biol Chem. 1985 Mar 25;260(6):3784-90.
3
The 36-kilodalton substrate of pp60v-src is myristylated in a transformation-sensitive manner.pp60v-src的36千道尔顿底物以一种对转化敏感的方式进行肉豆蔻酰化。
Science. 1985 Nov 1;230(4725):563-6. doi: 10.1126/science.2996139.
4
A ras-encoded protein in Dictyostelium discoideum is acylated and membrane-associated.盘基网柄菌中的一种由ras编码的蛋白质被酰化并与膜相关。
Mol Microbiol. 1987 Nov;1(3):347-54. doi: 10.1111/j.1365-2958.1987.tb01941.x.
5
Fatty acylation of heparan sulfate proteoglycan from human colon carcinoma cells.人结肠癌细胞硫酸乙酰肝素蛋白聚糖的脂肪酰化作用。
J Biol Chem. 1990 Nov 15;265(32):19980-9.
6
Myristoylation of proteins in the yeast secretory pathway.酵母分泌途径中蛋白质的豆蔻酰化作用。
J Biol Chem. 1992 Feb 25;267(6):3922-31.
7
African swine fever virus fatty acid acylated proteins.非洲猪瘟病毒脂肪酸酰化蛋白
Virology. 1991 Dec;185(2):942-5. doi: 10.1016/0042-6822(91)90578-y.
8
Myristic acid is incorporated into the two acylatable domains of the functional glycoprotein CD9 in ester, but not in amide bonds.肉豆蔻酸以酯键而非酰胺键的形式掺入功能性糖蛋白CD9的两个可酰化结构域中。
Biochim Biophys Acta. 1990 Jun 19;1039(2):218-26. doi: 10.1016/0167-4838(90)90189-m.
9
Acylation of proteins by myristic acid in isolated mitochondria.肉豆蔻酸在分离的线粒体中对蛋白质的酰化作用。
J Biol Chem. 1989 Apr 15;264(11):6376-80.
10
Chemical analysis of acylation sites and species.酰化位点和种类的化学分析。
Methods Enzymol. 1995;250:361-79. doi: 10.1016/0076-6879(95)50085-5.
引用本文的文献
1
N-myristoyltransferase.N-肉豆蔻酰转移酶
Mol Cell Biochem. 2000 Jan;204(1-2):135-55. doi: 10.1023/a:1007012622030.
2
Coenzyme A dependent myristoylation and demyristoylation in the regulation of bovine spleen N-myristoyltransferase.辅酶A依赖性肉豆蔻酰化与去肉豆蔻酰化对牛脾N-肉豆蔻酰转移酶的调控
Mol Cell Biochem. 1996 May 24;158(2):107-13. doi: 10.1007/BF00225835.
3
Mammalian myristoyl CoA: protein N-myristoyltransferase.哺乳动物肉豆蔻酰辅酶A：蛋白质N-肉豆蔻酰转移酶。
Mol Cell Biochem. 1995 Aug-Sep;149-150:191-202. doi: 10.1007/BF01076577.
4
Two distinct populations of ARF bound to Golgi membranes.两种不同的ARF群体与高尔基体膜结合。
J Cell Biol. 1993 May;121(4):751-60. doi: 10.1083/jcb.121.4.751.
5
Identification, purification and characterization of a membrane-associated N-myristoyltransferase inhibitor protein from bovine brain.
Biochem J. 1993 Apr 15;291 ( Pt 2)(Pt 2):635-9. doi: 10.1042/bj2910635.
6
Pheromone action regulates G-protein alpha-subunit myristoylation in the yeast Saccharomyces cerevisiae.信息素作用调节酿酒酵母中G蛋白α亚基的肉豆蔻酰化。
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9688-92. doi: 10.1073/pnas.90.20.9688.
7
Mechanisms of action of NIP71 on N-myristoyltransferase activity.NIP71对N-肉豆蔻酰转移酶活性的作用机制。
Mol Cell Biochem. 1994 Dec 21;141(2):79-86. doi: 10.1007/BF00926170.
本文引用的文献
1
In vivo acylation of Dictyostelium actin with palmitic acid.棕榈酸对活体粘菌肌动蛋白的酰化作用。
EMBO J. 1985 May;4(5):1153-6. doi: 10.1002/j.1460-2075.1985.tb03753.x.
2
Monoclonal antibodies against contact sites A of Dictyostelium discoideum: detection of modifications of the glycoprotein in tunicamycin-treated cells.针对盘基网柄菌接触位点 A 的单克隆抗体：衣霉素处理细胞中糖蛋白修饰的检测。
EMBO J. 1982;1(8):1011-6. doi: 10.1002/j.1460-2075.1982.tb01286.x.
3
Quantitative release of fatty acids from lipids by a simple hydrolysis procedure.通过简单的水解程序从脂质中定量释放脂肪酸。
J Lipid Res. 1983 Aug;24(8):1101-5.
4
Myristylation of gag-onc fusion proteins in mammalian transforming retroviruses.哺乳动物转化逆转录病毒中gag-onc融合蛋白的肉豆蔻酰化作用
Virology. 1984 Mar;133(2):431-7. doi: 10.1016/0042-6822(84)90409-4.
5
cAMP regulation of cell differentiation in Dictyostelium discoideum and the role of the cAMP receptor.盘基网柄菌中细胞分化的cAMP调节及cAMP受体的作用
Dev Biol. 1981 Apr 15;83(1):114-21. doi: 10.1016/s0012-1606(81)80013-9.
6
Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.细胞黏菌盘基网柄菌的黏变形体在无菌培养中的生长。
Biochem J. 1970 Sep;119(2):171-4. doi: 10.1042/bj1190171.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
Dynamics of antigenic membrane sites relating to cell aggregation in Dictyostelium discoideum.盘基网柄菌中与细胞聚集相关的抗原性膜位点的动态变化
J Cell Biol. 1973 Mar;56(3):647-58. doi: 10.1083/jcb.56.3.647.
9
Specificity of fatty acid acylation of cellular proteins.细胞蛋白质的脂肪酸酰化特异性
J Biol Chem. 1985 Mar 25;260(6):3784-90.
10
Acylation of cellular proteins with endogenously synthesized fatty acids.细胞蛋白质与内源性合成脂肪酸的酰化作用。
Biochemistry. 1986 Feb 25;25(4):878-84. doi: 10.1021/bi00352a021.
Zotero 插件