Raju R V, Magnuson B A, Sharma R K
Department of Pathology, College of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Canada.
Mol Cell Biochem. 1995 Aug-Sep;149-150:191-202. doi: 10.1007/BF01076577.
Myristoyl CoA:Protein N-myristoyltransferase (NMT) is the enzyme which catalyses the covalent transfer of myristate from myristoyl CoA to the amino-terminal glycine residue of protein substrates. Although NMT is ubiquitous in eukaryotic cells, the enzyme levels and cellular distribution vary among tissues. In this article, we describe the properties of mammalian NMT(s) with reference to subcellular distribution, molecular weights, substrate specificity and the possible involvement of NMT in pathological processes. The cytosolic fraction of bovine brain contains majority of NMT activity. In contrast, rabbit colon and rat liver NMT activity was predominantly particulate. Regional differences in NMT activity have been observed in both rabbit intestine and bovine brain. Results from our laboratory along with the existing knowledge, provide evidence for the existence of tissue specific isozymes of NMT.
肉豆蔻酰辅酶A：蛋白质N-肉豆蔻酰转移酶（NMT）是一种催化肉豆蔻酸从肉豆蔻酰辅酶A共价转移至蛋白质底物氨基末端甘氨酸残基的酶。尽管NMT在真核细胞中普遍存在，但酶水平和细胞分布在不同组织间存在差异。在本文中，我们参照亚细胞分布、分子量、底物特异性以及NMT在病理过程中可能的参与情况，描述了哺乳动物NMT的特性。牛脑的胞质部分含有大部分NMT活性。相比之下，兔结肠和大鼠肝脏的NMT活性主要存在于颗粒部分。在兔肠道和牛脑中均观察到了NMT活性的区域差异。我们实验室的结果以及现有知识为NMT组织特异性同工酶的存在提供了证据。
