临床实验室中自凝性O:11血清群气单胞菌菌株的分离与鉴定
Isolation and identification of autoagglutinating serogroup O:11 Aeromonas strains in the clinical laboratory.
作者信息
Kokka R P, Janda J M
机构信息
Microbial Diseases Laboratory, California Department of Health Services, Berkeley 94704.
出版信息
J Clin Microbiol. 1990 Jun;28(6):1297-9. doi: 10.1128/jcm.28.6.1297-1299.1990.
Abstract
We evaluated the extent to which serogroup O:11 Aeromonas strains could be recovered from both clinical and environmental specimens and the cultural parameters that affected the phenotypic marker (autoagglutination) associated with this group. Of over 200 Aeromonas strains screened, serogroup O:11 was identified only among the phenospecies A. hydrophila and A. sobria and was associated with clinical isolates more frequently than with environmental strains. Blood and wound isolates accounted for almost 50% of all O:11 strains identified. The autoagglutination phenotype associated with O:11 strains could be detected in most commercial liquid media, under a wide range of growth temperatures, and within 15 min of incubation at 100 degrees C. The results suggest that clinical laboratories can recognize this important group of Aeromonas strains by two simple tests.
摘要
我们评估了从临床和环境标本中分离出O:11血清群气单胞菌菌株的程度，以及影响该菌群相关表型标记（自身凝集）的培养参数。在筛选的200多种气单胞菌菌株中，仅在嗜水气单胞菌和温和气单胞菌表型种中鉴定出O:11血清群，且与临床分离株的关联比环境菌株更为频繁。血液和伤口分离株占所有鉴定出的O:11菌株的近50%。与O:11菌株相关的自身凝集表型可在大多数商业液体培养基中、在广泛的生长温度下以及在100℃孵育15分钟内检测到。结果表明，临床实验室可以通过两项简单测试识别这一重要的气单胞菌菌株群。
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