Beighton D, Whiley R A
Hunterian Dental Research Unit, London Hospital Medical College, Whitechapel, England.
J Clin Microbiol. 1990 Jun;28(6):1431-3. doi: 10.1128/jcm.28.6.1431-1433.1990.
Viridans group streptococci were examined for the production of sialidase (neuraminidase) activity, using the fluorescent substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid in a simple and rapid (15-min) assay. Sialidase was produced by all strains of Streptococcus oralis and S. intermedius and by a majority of S. mitis strains. S. mutans, S. sobrinus, S. gordonii, S. sanguis, S. vestibularis, S. salivarius, S. anginosus, S. constellatus, "S. parasanguis," and the "tufted fibril group" were uniformly negative. Sialidase production may be a useful characteristic to assist in the identification of viridans group streptococci.
利用荧光底物4-甲基伞形酮基-α-D-N-乙酰神经氨酸,通过一种简单快速(15分钟)的检测方法,对草绿色链球菌的唾液酸酶(神经氨酸酶)活性产生情况进行了检测。所有口腔链球菌和中间型链球菌菌株以及大多数缓症链球菌菌株都能产生唾液酸酶。变形链球菌、远缘链球菌、戈登链球菌、血链球菌、前庭链球菌、唾液链球菌、咽峡炎链球菌、星座链球菌、“副血链球菌”以及“簇状纤丝菌群”均呈一致阴性。唾液酸酶的产生可能是有助于鉴定草绿色链球菌的一个有用特征。