用于鉴定D群链球菌的快速乳胶凝集试验的评估
Evaluation of a rapid latex agglutination test for identification of group D streptococci.
作者信息
Vanzo S J, Washington J A
出版信息
J Clin Microbiol. 1984 Sep;20(3):575-6. doi: 10.1128/jcm.20.3.575-576.1984.
The SeroSTAT latex agglutination test (Scott Laboratories, Inc., Fiskesville, R.I.) for identification of group D streptococci was compared with bile-esculin agar and Lancefield grouping by using 110 clinical isolates of group D streptococci (S. faecalis, 74; S. bovis, 24; S. durans, 3; S. faecium, 9) and 65 viridans streptococci (S. anginosus-constellatus, 2; Streptococcus MG, 15; S. sanguis II, 14; S. mitis, 7; S. mutans, 5; S. salivarius, 8; S. sanguis I, 8; S. acidominimus, 1; S. morbillorum, 2; S. pneumoniae, 3). All strains of group D streptococci were bile-esculin positive. SeroSTAT reactions were falsely positive with 2 strains of S. sanguisII and 1 strain of Streptococcus MG (4.6% of all viridans streptococci tested) and falsely negative with 10 S. bovis, 8 S. faecalis, 1 S. durans, and 6 S. faecium strains (22.7% of all group D streptococci tested). When considered with colonial morphology and hemolytic reaction, SeroSTAT is a rapid (60-s) and useful test for recognition of group D streptococci.
采用110株D群链球菌（粪肠球菌74株、牛链球菌24株、耐久链球菌3株、屎肠球菌9株）和65株草绿色链球菌（咽峡炎链球菌-星座链球菌2株、MG链球菌15株、血链球菌II 14株、缓症链球菌7株、变形链球菌5株、唾液链球菌8株、血链球菌I 8株、嗜酸链球菌1株、麻疹链球菌2株、肺炎链球菌3株），将用于鉴定D群链球菌的SeroSTAT乳胶凝集试验（Scott Laboratories公司，罗德岛州菲克斯维尔）与胆汁七叶苷琼脂试验和兰斯菲尔德血清学分型法进行比较。所有D群链球菌菌株胆汁七叶苷试验均为阳性。SeroSTAT试验对2株血链球菌II和1株MG链球菌出现假阳性反应（占所有检测草绿色链球菌的4.6%），对10株牛链球菌、8株粪肠球菌、1株耐久链球菌和6株屎肠球菌出现假阴性反应（占所有检测D群链球菌的22.7%）。结合菌落形态和溶血反应来看，SeroSTAT是一种快速（60秒）且有助于识别D群链球菌的试验。
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