两种用于鉴定流感嗜血杆菌并进行生物分型的快速方法的评估
Evaluation of two rapid methods for identifying and biotyping Haemophilus influenzae.
作者信息
Murphy P G, Craig I, Lafong A C, Smyth E T
机构信息
Department of Bacteriology, Belfast City Hospital, Northern Ireland.
出版信息
J Clin Pathol. 1990 Jul;43(7):581-3. doi: 10.1136/jcp.43.7.581.
Several rapid method kits (one to four hours) have become available for the identification of Haemophilus and related genera. Two kits (the "Rapid NH" system and the "RIM Haemophilus" system), which include the identification and biotyping of H influenzae, were investigated for the rapid identification and biotyping of 193 isolates of H influenzae and the results compared with those obtained by more standard overnight methods. The kits were convenient to use and gave reliable and rapid speciation of all isolates. Both test systems were unreliable for biotyping: 42 isolates were wrongly biotyped by the RIM kit and 40 isolates wrongly biotyped by the rapid NH kit. It is concluded that the test kits may be useful for the rapid identification of H influenzae but that they are not reliable for the biotyping of this species.
已有几种快速检测试剂盒（1至4小时）可用于鉴定嗜血杆菌及相关菌属。对两种试剂盒（“快速NH”系统和“RIM嗜血杆菌”系统）进行了研究，这两种试剂盒包括流感嗜血杆菌的鉴定和生物分型，用于对193株流感嗜血杆菌进行快速鉴定和生物分型，并将结果与通过更标准的过夜培养方法获得的结果进行比较。这些试剂盒使用方便，能对所有分离株进行可靠且快速的菌种鉴定。两种检测系统在生物分型方面都不可靠：RIM试剂盒对42株分离株生物分型错误，快速NH试剂盒对40株分离株生物分型错误。结论是，这些检测试剂盒可能有助于流感嗜血杆菌的快速鉴定，但对该菌种的生物分型不可靠。
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