通过乳胶凝集法对脑膜炎球菌和包膜流感嗜血杆菌菌株进行血清学分型。
Serological grouping of meningococci and encapsulated Haemophilus influenzae strains by latex agglutination.
作者信息
Leinonen M, Sivonen A
出版信息
J Clin Microbiol. 1979 Oct;10(4):404-8. doi: 10.1128/jcm.10.4.404-408.1979.
Abstract
The latex agglutination method, utilizing antibody-coated latex particles, was adapted for serogrouping of Neisseria meningitidis and serotyping of encapsulated Haemophilus influenzae strains from agar plates. It was found to give more clear-cut results than conventional slide agglutination. A 100% agreement with the antiserum agar method was found for all strains isolated from blood or cerebrospinal fluid. Many meningococcal strains from nasopharyngeal carriers are autoagglutinable, but some of these gave a positive reaction with the group B latex reagent, although they were negative by the antiserum agar method. The latex agglutination method has several advantages over others: the lack of autoagglutination, easy performance, easy interpretation, and very low consumption of antisera.
摘要
利用包被抗体的乳胶颗粒的乳胶凝集法，被用于从琼脂平板上对脑膜炎奈瑟菌进行血清群分型以及对有荚膜的流感嗜血杆菌菌株进行血清分型。结果发现，该方法比传统玻片凝集法能给出更明确的结果。从血液或脑脊液中分离出的所有菌株，该方法与抗血清琼脂法的结果完全一致。许多来自鼻咽部携带者的脑膜炎球菌菌株可自身凝集，但其中一些与B群乳胶试剂呈阳性反应，尽管用抗血清琼脂法检测为阴性。乳胶凝集法相对于其他方法有几个优点：无自身凝集现象、操作简便、结果易判读且抗血清消耗极低。
相似文献
1
Serological grouping of meningococci and encapsulated Haemophilus influenzae strains by latex agglutination.通过乳胶凝集法对脑膜炎球菌和包膜流感嗜血杆菌菌株进行血清学分型。
J Clin Microbiol. 1979 Oct;10(4):404-8. doi: 10.1128/jcm.10.4.404-408.1979.
2
Methods for serotyping nasopharyngeal isolates of Haemophilus influenzae: slide agglutination, Quellung reaction, countercurrent immunoelectrophoresis, latex agglutination, and antiserum agar.流感嗜血杆菌鼻咽分离株的血清分型方法：玻片凝集法、荚膜肿胀反应、对流免疫电泳、乳胶凝集法和抗血清琼脂法。
J Clin Microbiol. 1979 May;9(5):570-4. doi: 10.1128/jcm.9.5.570-574.1979.
3
Serogroup identification of Neisseria meningitidis: comparison of an antiserum agar method with bacterial slide agglutination.脑膜炎奈瑟菌血清群鉴定：抗血清琼脂法与细菌玻片凝集法的比较
J Clin Microbiol. 1978 May;7(5):410-4. doi: 10.1128/jcm.7.5.410-414.1978.
4
Modified latex agglutination test for rapid detection of Streptococcus pneumoniae and haemophilus influenzae in cerebrospinal fluid and direct serotyping of Streptococcus pneumoniae.改良乳胶凝集试验用于快速检测脑脊液中的肺炎链球菌和流感嗜血杆菌以及肺炎链球菌的直接血清分型。
Eur J Clin Microbiol Infect Dis. 1996 Jun;15(6):472-7. doi: 10.1007/BF01691314.
5
Serogrouping of non-interpretable Neisseria meningitidis carriage strains, using rapid diagnostic tests.使用快速诊断检测对不可判读的脑膜炎奈瑟菌携带菌株进行血清分型
Trans R Soc Trop Med Hyg. 2008 Jan;102(1):99-100. doi: 10.1016/j.trstmh.2007.09.007. Epub 2007 Oct 31.
6
Latex agglutination test for screening of Haemophilus influenzae type b carriers.用于筛查 b 型流感嗜血杆菌携带者的乳胶凝集试验。
J Clin Microbiol. 1987 Jun;25(6):1131-3. doi: 10.1128/jcm.25.6.1131-1133.1987.
7
Evaluation of bactericidal assay in serotyping Neisseria meningitidis group A.用于A群脑膜炎奈瑟菌血清分型的杀菌试验评估
Can J Microbiol. 1979 Aug;25(8):929-31. doi: 10.1139/m79-139.
8
Use of antiserum agar plates for serogrouping of meningococci.使用抗血清琼脂平板对脑膜炎球菌进行血清分组。
J Clin Pathol. 1977 Sep;30(9):834-7. doi: 10.1136/jcp.30.9.834.
9
Comparison of methods for serotyping isolates of Haemophilus influenzae.流感嗜血杆菌分离株血清分型方法的比较
J Clin Microbiol. 1985 Feb;21(2):158-60. doi: 10.1128/jcm.21.2.158-160.1985.
10
The latex agglutination test for the diagnosis of meningococcal and haemophilus influenzae meningitis.用于诊断脑膜炎球菌性和流感嗜血杆菌性脑膜炎的乳胶凝集试验。
Scand J Infect Dis. 1977;9(3):187-91. doi: 10.3109/inf.1977.9.issue-3.06.
引用本文的文献
1
International circumpolar surveillance interlaboratory quality control program for serotyping Haemophilus influenzae and serogrouping Neisseria meningitidis, 2005 to 2009.2005 年至 2009 年，国际极地区域流感嗜血杆菌血清分型和脑膜炎奈瑟菌血清群监测的实验室间质量控制计划。
J Clin Microbiol. 2012 Mar;50(3):651-6. doi: 10.1128/JCM.05084-11. Epub 2011 Dec 14.
2
Detection of Pneumococcal Capsular polysaccharide antigens by latex agglutination, counterimmunoelectrophoresis, and radioimmunoassay in middle ear exudates in acute otitis media.通过乳胶凝集试验、对流免疫电泳和放射免疫测定法检测急性中耳炎中耳渗出液中的肺炎球菌荚膜多糖抗原。
J Clin Microbiol. 1980 Feb;11(2):135-40. doi: 10.1128/jcm.11.2.135-140.1980.
3
P-fimbriated clones among uropathogenic Escherichia coli strains.尿路致病性大肠杆菌菌株中的P菌毛克隆
Infect Immun. 1984 Jan;43(1):149-55. doi: 10.1128/iai.43.1.149-155.1984.
4
Simple latex agglutination method for typing pneumococci.肺炎球菌分型的简易乳胶凝集法。
J Clin Pathol. 1988 Feb;41(2):230-1. doi: 10.1136/jcp.41.2.230.
5
Evaluation of two rapid methods for identifying and biotyping Haemophilus influenzae.两种用于鉴定流感嗜血杆菌并进行生物分型的快速方法的评估
J Clin Pathol. 1990 Jul;43(7):581-3. doi: 10.1136/jcp.43.7.581.
本文引用的文献
1
THE AETIOLOGY OF ACUTE MIDDLE EAR INFECTION.急性中耳感染的病因学
Acta Otolaryngol. 1964 Aug-Sep;58:149-58. doi: 10.3109/00016486409121372.
2
A bacteriologic investigation of otitis media in infancy.婴儿期中耳炎的细菌学调查。
Pediatrics. 1956 Mar;17(3):359-67.
3
An Escherichia coli antigen cross-reactive with the capsular polysaccharide of Haemophilus influenzae type b: occurrence among known serotypes, and immunochemical and biologic properties of E. coli antisera toward H. influenzae type b.一种与b型流感嗜血杆菌荚膜多糖发生交叉反应的大肠杆菌抗原：在已知血清型中的出现情况，以及大肠杆菌抗血清针对b型流感嗜血杆菌的免疫化学和生物学特性。
J Immunol. 1972 Jun;108(6):1551-62.
4
Frequency of typable and non-typable Haemophilus influenzae strains in children with acute otitis media and results of penicillin V treatment.急性中耳炎患儿中可分型和不可分型流感嗜血杆菌菌株的频率及青霉素V治疗结果
Scand J Infect Dis. 1971;3(3):225-8. doi: 10.3109/inf.1971.3.issue-3.08.
5
Counter-current immunoelectrophoresis in the diagnosis of meningococcal infections.对流免疫电泳在脑膜炎球菌感染诊断中的应用
Lancet. 1971 Sep 4;2(7723):519-21. doi: 10.1016/s0140-6736(71)90439-9.
6
Bacterial antigens cross-reactive with the capsular polysaccharide of Haemophilus influenzae type b.与b型流感嗜血杆菌荚膜多糖交叉反应的细菌抗原。
Lancet. 1971 May 29;1(7709):1095-6. doi: 10.1016/s0140-6736(71)91837-x.
7
The use of protein A-containing staphylococci sensitized with anti-meningococcal antibodies for grouping Neisseria meningitidis and demonstration of meningococcal antigen in cerebrospinal fluid.使用用抗脑膜炎球菌抗体致敏的含蛋白A葡萄球菌对脑膜炎奈瑟菌进行分组，并在脑脊液中检测脑膜炎球菌抗原。
Acta Pathol Microbiol Scand B. 1975 Aug;83(4):387-96. doi: 10.1111/j.1699-0463.1975.tb00117.x.
8
Serological grouping of meningococci and detection of antigen in cerebrospinal fluid by coagglutination.
Med Lab Sci. 1978 Jan;35(1):63-6.
9
A comparison of the serum agar grouping and slide agglutination methods for the identification of Neisseria meningitidis strains.用于鉴定脑膜炎奈瑟菌菌株的血清琼脂分组法与玻片凝集法的比较。
Bull World Health Organ. 1977;55(6):659-61.
10
Use of antiserum agar plates for serogrouping of meningococci.使用抗血清琼脂平板对脑膜炎球菌进行血清分组。
J Clin Pathol. 1977 Sep;30(9):834-7. doi: 10.1136/jcp.30.9.834.
Zotero 插件