Division of Fish Genetics and Biotechnology, Indian Council of Agricultural Research (ICAR)-Central Institute of Fisheries Education Mumbai, Mumbai, India.
Faculty of Life and Environmental Sciences, University of Iceland, Reykjavik, Iceland.
Front Endocrinol (Lausanne). 2022 Jun 30;13:899463. doi: 10.3389/fendo.2022.899463. eCollection 2022.
In the present work the nonapeptides i.e., isotocin and vasotocin alone or in a combination were tested in to evaluate their effect on stripping by abdominal massage. Also, we used chitosan-carbon nanotube nanocomposites to conjugate the nonapetides isotocin (abbreviated as COOH-SWCNTCSPeP) and isotocin and vasotocin (COOH-SWCNTCSPePs) with the aim of sustaining the effect for a longer duration. The conjugation of nonapeptides with nanocomposites was confirmed by Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS). Two experiments were conducted to study the effect of naked (without nanoparticles) and conjugated nonapeptides on the milt release by stripping. Both the experiments consisted of eight treatments which included four naked groups two nanoconjugated groups and two controls. Both naked and nonconjugated formulations were successful in stripping the male catfish. The mRNA expression of selected reproductive genes was analysed to decipher the effect of nanopeptides at the molecular level. Nonapeptide treatment either naked or nanoconjugated, resulted in the upregulation of the transcript level of genes. Histological analysis revealed the concentration of spermatozoa was more in peptide injected groups than in the controls. The synergistic effects of nonapeptides and Ovatide had a positive impact on GSI. Thus, the present formulations were successful in stripping the male catfish to obtain the milt with significant reproductive success. Even though the naked groups perform better but the number of males required to fertilize the eggs in nanoconjuagted groups was smaller making it worth using for the delivery of nonapeptides.
在本工作中，单独或组合使用九肽即催产素和血管加压素，以评估其对腹部按摩剥离子的影响。此外，我们还使用壳聚糖-碳纳米管纳米复合材料来缀合九肽催产素（缩写为 COOH-SWCNTCSPeP）和催产素和血管加压素（COOH-SWCNTCSPePs），旨在延长其作用持续时间。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、热重分析（TGA）和 X 射线光电子能谱（XPS）证实了非肽与纳米复合材料的缀合。进行了两项实验以研究裸肽（无纳米粒子）和缀合非肽对剥离时精液释放的影响。这两项实验都包含八个处理，包括四个裸肽组、两个纳米缀合组和两个对照。裸肽和非缀合配方都成功地剥离了雄性鲶鱼。分析了所选生殖基因的 mRNA 表达，以从分子水平上阐明纳米肽的作用。非肽处理无论是裸肽还是纳米缀合，都会导致基因转录水平的上调。组织学分析显示，注射肽的组中的精子浓度高于对照组。非肽和 Ovaitide 的协同作用对 GSI 有积极影响。因此，本配方成功地剥离了雄性鲶鱼，获得了具有显著生殖成功的精液。尽管裸肽组表现更好，但在纳米缀合组中所需的雄性数量较少，这使其在非肽的递送方面更有价值。
