Department of Chemistry, Sharif University of Technology, Tehran, Iran.
Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, 14155-6451, Tehran, Iran.
Sci Rep. 2021 Mar 23;11(1):6604. doi: 10.1038/s41598-021-86119-z.
Herein, in a one-pot method, the reduced graphene oxide layers with the assistance of multiwalled carbon nanotubes were decorated to provide a suitable space for the in situ growth of CoNiS, and the porphyrins were incorporated into the layers as well to increase the sensitivity of the prepared nanostructure. The prepared nanocomposite can establish π-π interactions between the genetic material and on the surface of porphyrin rings. Also, hydrogen bonds between genetic domains and the porphyrin' nitrogen and the surface hydroxyl groups are probable. Furthermore, the potential donor-acceptor relationship between the d transition metal, cobalt, and the genetic material provides a suitable way to increase the interaction and gene loading , and transfections. The reason for this phenomenon was optimized to increase the EGFP by up to 17.9%. Furthermore, the sensing ability of the nanocomposite towards HO was investigated. In this regard, the limit of detection of the HO obtained 10 µM. Also, the in situ biosensing ability in the HEK-293 and PC12 cell lines was evaluated by the addition of PMA. The nanocomposite showed the ability to detect the released HO after adding the minimum amount of 120 ng/mL of the PMA.
在此，通过一锅法，在还原氧化石墨烯层上修饰了多壁碳纳米管，为 CoNiS 的原位生长提供了合适的空间，同时将卟啉也掺入到层中，以提高所制备的纳米结构的灵敏度。所制备的纳米复合材料可以在遗传物质和卟啉环表面之间建立π-π相互作用。此外，遗传结构域与卟啉的氮和表面羟基之间可能存在氢键。此外，d 过渡金属钴与遗传物质之间的潜在供体-受体关系为增加相互作用和基因负载以及转染提供了合适的途径。优化了这一现象的原因，使 EGFP 增加了 17.9%。此外，还研究了纳米复合材料对 HO 的传感能力。在这方面，HO 的检出限达到了 10 μM。此外，还通过添加 PMA 评估了纳米复合材料在 HEK-293 和 PC12 细胞系中的原位生物传感能力。纳米复合材料显示出在添加最小量 120ng/mL 的 PMA 后检测释放的 HO 的能力。
