Department of Dermatology, Peking University First Hospital, and Research Center for Medical Mycology, Peking University, Beijing, China.
Mycoses. 2011 Oct;54 Suppl 3:5-11. doi: 10.1111/j.1439-0507.2011.02108.x.
The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.
PCR-RLB（反向线杂交）被应用于从囊性纤维化（CF）患者的痰液中检测假丝酵母属和枝孢属成员的分子技术。从 52 名 CF 患者中采集了 59 份痰液样本，通过培养和 PCR-RLB 进行分析。常规和半选择性培养得到了 5 个阳性样本，但 PCR-RLB 杂交检测法允许在 52 名患者中的 32 名（61.5%）中检测到假丝酵母属/枝孢属成员。总共，PCR-RLB 产生了 47 个阳性结果。假丝酵母属在 20 个样本中被检测到，而假丝酵母属和黄曲霉属分别在 17 个和 8 个样本中被检测到。6 个样本与两种不同的种特异性探针呈阳性反应，1 个样本与 3 种探针呈阳性反应。总之，本研究中描述的 PCR-RLB 检测法允许在 CF 痰液样本中检测枝孢属 spp.，并鉴定出假丝酵母属、假丝酵母属、黄曲霉属、 prolificans 和 Pseudallescheria minutispora。
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