Exposure and Biomonitoring Division, Health Canada, Ottawa, Ontario, Canada.
Anal Chim Acta. 2011 Nov 7;706(1):176-83. doi: 10.1016/j.aca.2011.07.040. Epub 2011 Aug 27.
Haloacetic acids (HAAs) are by-products of the chlorination of drinking water containing natural organic matter and bromide. A simple and sensitive method has been developed for determination of ten HAAs in drinking water. The pressure-assisted electrokinetic injection (PAEKI), an on-line enrichment technique, was employed to introduce the sample into a capillary electrophoresis (CE)-electrospray ionization-tandem mass spectrometry system (ESI-MS/MS). HAAs were monitored in selected reaction monitoring mode. With 3 min of PAEKI time, the ten major HAAs (HAA10) in drinking water were enriched up to 20,000-fold into the capillary without compromising resolution. A simple solid phase clean-up method has been developed to eliminate the influence of ionic matrices from drinking water on PAEKI. Under conditions optimized for mass spectrometry, PAEKI and capillary electrophoresis, detection limits defined as three times ratio of signal to noise have been achieved in a range of 0.013-0.12 μg L(-1) for ten HAAs in water sample. The overall recoveries for all ten HAAs in drinking water samples were between 76 and 125%. Six HAAs including monochloro- (MCAA), dichloro- (DCAA), trichloro- (TCAA), monobromo- (MBAA), bromochloro- (BCAA), and bromodichloroacetic acids (BDCAA) were found in tap water samples collected.
卤乙酸（HAAs）是含有天然有机物和溴化物的饮用水氯化的副产物。本文建立了一种简单、灵敏的测定饮用水中十种卤乙酸的方法。采用压力辅助电动进样（PAEKI）作为在线浓缩技术，将样品引入毛细管电泳（CE）-电喷雾串联质谱（ESI-MS/MS）系统。采用选择反应监测模式对 HAAs 进行监测。通过 3 min 的 PAEKI 时间，可以将饮用水中的十种主要 HAAs（HAA10）浓缩至 20,000 倍，而不会影响分辨率。建立了一种简单的固相萃取净化方法，以消除饮用水中离子基质对 PAEKI 的影响。在优化质谱和毛细管电泳条件下，十种 HAAs 在水样中的检测限定义为信号与噪声的 3 倍，范围为 0.013-0.12μg/L。十种 HAAs 在饮用水样品中的总回收率在 76%至 125%之间。在采集的自来水样品中发现了六种 HAAs，包括一氯乙酸（MCAA）、二氯乙酸（DCAA）、三氯乙酸（TCAA）、一溴乙酸（MBAA）、溴氯乙酸（BCAA）和二溴二氯乙酸（BDCAA）。
