Department of Physics and Astrophysics, University of Delhi, India.
Anal Chim Acta. 2012 May 13;726:93-101. doi: 10.1016/j.aca.2012.03.027. Epub 2012 Mar 22.
RF sputtered p-type nickel oxide (NiO) thin film exhibiting tunable semiconductor character which in turns enhanced its functional properties. NiO thin film with high hole mobility is developed as a potential matrix for the realization of glucose biosensor. NiO thin film prepared under the optimized deposition conditions offer good electrical conductivity (1.5×10(-3) Ω(-1)-cm(-1)) with high hole mobility (2.8 cm(2) V(-1) s(-1)). The bioelectrode (GO(x)/NiO/ITO/glass) exhibits a low value of Michaelis-Menten constant (K(m)=1.05 mM), indicating high affinity of the immobilized GO(x) toward the analyte (glucose). Due to the high surface coverage (2.32×10(-7) mol cm(-2)) of the immobilized enzyme on to the NiO matrix and its high electrocatalytic activity, the prepared biosensor exhibits a high sensitivity of 0.1 mA(mM(-1)-cm(-2)) and a good linearity from 25 to 300 mg dL(-1) of glucose concentration with fast response time of 5 s. Various functional properties of the material (mobility, crystallinity and stress) are found to influence the charge communication feature of NiO thin film matrix to a great extent, resulting in enhanced sensing response characteristics.
射频磁控溅射制备的 p 型镍氧化物（NiO）薄膜具有可调半导体特性，从而增强了其功能特性。具有高空穴迁移率的 NiO 薄膜被开发为实现葡萄糖生物传感器的潜在基质。在优化的沉积条件下制备的 NiO 薄膜具有良好的电导率（1.5×10(-3) Ω(-1)-cm(-1))和高空穴迁移率（2.8 cm(2) V(-1) s(-1))。生物电极（GO(x)/NiO/ITO/glass）表现出低米氏常数（K(m)=1.05 mM），表明固定化 GO(x)对分析物（葡萄糖）具有高亲和力。由于固定化酶在 NiO 基质上的高表面覆盖率（2.32×10(-7) mol cm(-2))和高电催化活性，制备的生物传感器表现出 0.1 mA(mM(-1)-cm(-2))的高灵敏度和 25 至 300 mg dL(-1)葡萄糖浓度的良好线性关系，响应时间快至 5 s。材料的各种功能特性（迁移率、结晶度和应力）被发现极大地影响 NiO 薄膜基质的电荷传递特性，从而增强了传感响应特性。
