Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
J Dent. 2012 Jan;40(1):41-7. doi: 10.1016/j.jdent.2011.09.009. Epub 2011 Oct 1.
To investigate the potential of an active attachment biofilm model as a high-throughput demineralization biofilm model for the evaluation of caries-preventive agents.
Streptococcus mutans UA159 biofilms were grown on bovine dentine discs in a high-throughput active attachment model. Biofilms were first formed in a medium with high buffer capacity for 24h and then subjected to various photodynamic therapies (PACT) using the combination of Light Emitting Diodes (LEDs, Biotable(®)) and Photogem(®). Viability of the biofilms was evaluated by plate counts. To investigate treatment effects on dentine lesion formation, the treated biofilms were grown in a medium with low buffer capacity for an additional 24h. Integrated mineral loss (IML) and lesion depth (LD) were assessed by transversal microradiography. Calcium release in the biofilm medium was measured by atomic absorption spectroscopy.
Compared to the water treated control group, significant reduction in viability of S. mutans biofilms was observed when the combination of LEDs and Photogem(®) was applied. LEDs or Photogem(®) only did not result in biofilm viability changes. Similar outcomes were also found for dentine lesion formation. Significant lower IML and LD values were only found in the group subjected to the combined treatment of LEDs and Photogem(®). There was a good correlation between the calcium release data and the IML or LD values.
The high-throughput active attachment biofilm model is applicable for evaluating novel caries-preventive agents on both biofilm and demineralization inhibition. PACT had a killing effect on 24h S. mutans biofilms and could inhibit the demineralization process.
研究活性附着生物膜模型作为一种高通量脱矿生物膜模型，用于评估防龋剂的潜在应用。
在高通量活性附着模型中，将变形链球菌 UA159 生物膜培养在牛牙本质片上。生物膜首先在高缓冲能力的培养基中形成 24 小时，然后用发光二极管（LED，Biotable(®)）和 Photogem(®)联合的各种光动力疗法（PACT）进行处理。通过平板计数评估生物膜的存活率。为了研究治疗对牙本质病变形成的影响，将处理后的生物膜在低缓冲能力的培养基中再培养 24 小时。通过横切显微放射摄影评估整合矿物损失（IML）和病变深度（LD）。通过原子吸收光谱法测量生物膜培养基中的钙释放。
与水治疗对照组相比，当应用 LED 和 Photogem(®)联合时，变形链球菌生物膜的存活率显著降低。单独使用 LED 或 Photogem(®)不会导致生物膜存活率发生变化。牙本质病变形成也出现了类似的结果。仅在接受 LED 和 Photogem(®)联合治疗的组中发现 IML 和 LD 值显著降低。钙释放数据与 IML 或 LD 值之间存在良好的相关性。
高通量活性附着生物膜模型适用于评估新型防龋剂在生物膜和脱矿抑制方面的应用。PACT 对 24 小时变形链球菌生物膜具有杀伤作用，并能抑制脱矿过程。
