Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Alameda Dr. Octavio Pinheiro Brisolla, 9-75, Vila Universitária, Bauru, São Paulo, 17012-901, Brazil.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
Lasers Med Sci. 2019 Aug;34(6):1185-1192. doi: 10.1007/s10103-018-02708-x. Epub 2019 Jan 2.
To our knowledge, there is still no evidence in relation to the combination of curcumin with chelants to improve the effects of antimicrobial photodynamic therapy (aPDT) on complex dental caries biofilms. Therefore, the aim of this study was to evaluate the antimicrobial effect of curcumin-ethylenediaminetetraacetic acid (EDTA)-mediated aPDT on the vitality of intact biofilms of dentin caries microcosms. Biofilms were grown on glass slabs in McBain medium plus 1% sucrose in microaerophily at 37 °C for 5 days. Then, biofilms were treated with associations of 600 μmol L curcumin combined or not with 1% EDTA and 37.5 or 75 J cm LED (455 nm). The vitality was determined by a confocal laser scanning microscopy (CLSM) after staining biofilms with a mixture of 2.5 g L fluorescein diacetate and 0.25 g L ethidium bromide. Statistical analysis was conducted by Kruskal-Wallis and post hoc Dunn's test (P < 0.05). Three treatments were able to reduce the vitality of overall biofilms: curcumin + 75 J cm LED, curcumin-EDTA + 37.5 J cm LED, and curcumin-EDTA + 75 J cm LED. Also, the vitality of inner layers of biofilms was significantly reduced only after the combination of aPDT with EDTA. Therefore, the association of curcumin and EDTA improved the antimicrobial effect of aPDT on dentin caries microcosms, considering the application of lower light densities and deeper layers of biofilms.
据我们所知，目前尚无证据表明姜黄素与螯合剂联合使用可以提高抗菌光动力疗法（aPDT）对复杂牙本质龋生物膜的疗效。因此，本研究旨在评估姜黄素-乙二胺四乙酸（EDTA）介导的 aPDT 对牙本质龋微宇宙完整生物膜活力的抗菌作用。生物膜在微需氧条件下于 McBain 培养基中加 1%蔗糖于 37°C 培养 5 天，在玻璃片上生长。然后，用 600μmol/L 姜黄素联合或不联合 1% EDTA 和 37.5 或 75J/cm2 LED（455nm）处理生物膜。用 2.5g/L 荧光素二乙酸酯和 0.25g/L 溴化乙锭的混合物对生物膜进行染色后，通过共聚焦激光扫描显微镜（CLSM）确定活力。采用 Kruskal-Wallis 和事后 Dunn 检验（P<0.05）进行统计学分析。三种处理方法均能降低整体生物膜的活力：姜黄素+75J/cm2 LED、姜黄素-EDTA+37.5J/cm2 LED 和姜黄素-EDTA+75J/cm2 LED。此外，只有在 aPDT 与 EDTA 联合使用后，生物膜内层的活力才会显著降低。因此，考虑到较低的光密度和生物膜更深层的应用，姜黄素和 EDTA 的联合使用提高了 aPDT 对牙本质龋微宇宙的抗菌效果。
