Department of Veterinary Clinical Sciences, Università degli Studi di Teramo, Viale Crispi 212, Teramo, Italy.
Anim Reprod Sci. 2011 Oct;128(1-4):117-22. doi: 10.1016/j.anireprosci.2011.09.007. Epub 2011 Sep 25.
Spermatozoa acquire their motility and fertilizing ability during their passage through the epididymal canal. In the epididymal caput and corpus spermatozoa undergo several biochemical and metabolic changes while the cauda of the epididymis should be considered as the primarily site for storage of the spermatozoa. In the horse spermatozoa from cauda epididymis were collected and frozen, and the fertility of semen assessed. However, no studies have detailed semen characteristics of spermatozoa collected from the cauda epididymis in the jackass. In this study sperm characteristics of spermatozoa in the cauda epididymis of the donkey was reported and a comparison with ejaculated spermatozoal characteristics was performed. Samples from 10 Martina Franca jackasses were collected and analyzed for viability (Propidium iodide/Sybr-14® fluorescent stain), mitochondrial activity (Mitotraker® fluorescent stain), objective motility characteristics (by Computer Assisted Sperm Analyzer - CASA) and morphology. A higher viability and mitochondrial activity in the cauda epididymis samples were reported in this paper. Samples reported in this paper were identified and the percentage of total and progressive spermatozoa was comparable, but trajectories were more rapid (higher VCL) with less progressiveness (higher ALH and lower STR and LIN) in the cauda epididymis. Sperm morphology showed a pronounced variability between jackasses, with comparable values for all morphological subclasses. In this study the loss of the distal cytoplasmic droplets happen close to or after ejaculation because the percentage fell to nearly 0% after ejaculation. As suggested for bulls, the presence of a similar percentage in sperm with proximal cytoplasmic droplet in epididymal and ejaculated semen is likely to indicate a failure in the maturation process.
精子在通过附睾管的过程中获得运动能力和受精能力。在附睾头和体部，精子经历了几种生化和代谢变化，而附睾尾应被视为精子的主要储存部位。在马中，从附睾尾收集精子并进行冷冻，然后评估精液的受精能力。然而，尚无研究详细描述驴附睾尾精子的精液特征。在这项研究中，报告了驴附睾尾精子的特征，并与射出精子的特征进行了比较。从 10 头 Martina Franca 驴中采集并分析了 10 头驴的精子活力（碘化丙啶/Sybr-14®荧光染色）、线粒体活性（Mitotraker®荧光染色）、客观运动特性（计算机辅助精子分析 - CASA）和形态。本文报道了附睾尾样本的活力和线粒体活性较高。本文报告的样本被鉴定，总精子和前向运动精子的百分比是可比的，但轨迹在附睾尾更快（更高的 VCL），前向性更低（更高的 ALH 和更低的 STR 和 LIN）。精子形态在驴之间表现出明显的可变性，所有形态子类别的值都相当。在这项研究中，由于射出后百分比接近或降至 0%，因此远端细胞质滴的丢失发生在接近射出或射出后。正如对公牛的建议一样，在附睾和射出精液中具有近端细胞质滴的精子中存在类似的百分比可能表明成熟过程失败。
