取代基对三联吡啶钌(II)配合物与 DNA 相互作用的影响。
Effect of substituent of terpyridines on the DNA-interaction of polypyridyl ruthenium(II) complexes.
机构信息
Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar, Gujarat, India.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Dec 15;84(1):243-8. doi: 10.1016/j.saa.2011.09.037. Epub 2011 Sep 22.
An octahedral complexes of ruthenium with 2,9-dimethyl-1,10-phenanthroline (dmphen) and substituted terpyridine have been synthesized. The Ru(II) complexes have been characterized by elemental analyses, thermogravimetric analyses, magnetic moment measurements, FT-IR, electronic, (1)H NMR and FAB mass spectra. The binding strength and mode of interaction of the complexes with Herring Sperm DNA has been investigated using absorption titration and viscosity measurement studies. Results suggest that the substituent on terpyridine ligand affects the binding mode and binding ability of the complexes. Effect of time and ionic strength on DNA cleavage ability of complex has also been studied by gel electrophoresis. Results suggest that more than 200 mM concentration of NaCl decreases the cleavage ability of complex.
已合成了具有 2,9-二甲基-1,10-菲啰啉（dmphen）和取代的三联吡啶的钌（II）的八面体配合物。Ru（II）配合物通过元素分析、热重分析、磁矩测量、FT-IR、电子、（1）H NMR 和 FAB 质谱进行了表征。通过吸收滴定和粘度测量研究，研究了配合物与鲱精 DNA 的结合强度和相互作用模式。结果表明，三联吡啶配体上的取代基影响配合物的结合模式和结合能力。还通过凝胶电泳研究了时间和离子强度对配合物切割 DNA 能力的影响。结果表明，NaCl 的浓度超过 200 mM 会降低配合物的切割能力。
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