Udomkusonsri Pareeya, Kaewmokul Santi, Arthitvong Surapong, Phaochoosak Napasorn
Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
J Vet Med Sci. 2012 Mar;74(3):301-5. doi: 10.1292/jvms.11-0095. Epub 2011 Oct 14.
Antibiotic beads have become popular for the treatment of local bacterial infections. The preparation of antibiotic beads from commercial pharmaceutical antibiotics is a convenient method in clinic. The elution characteristics of cefazolin from polymethylmethacrylate (PMMA) (SmartSet HV, Depuy I and Cemfix 3) beads and calcium sulfate beads were studied. Commercial cefazolin formulation was incorporated in PMMA or calcium sulfate at 1 g cefazolin/10 g of matrix substances to form beads. The concentrations of eluted cefazolin during 15 days were greater than MIC for Staphylococcus aureus (ATCC 25923). The eluted cefazolin concentrations were in the range of 3.6 ± 1.2 to 4.6 ± 0.4 mg for PMMA beads and 15.4 ± 1.7 mg for calcium sulfate beads. The accumulated eluted cefazolin from PMMA beads and calcium sulfate beads for 15 days were 34.41 ± 3.93 to 38.67 ± 3.04% and 95.94 ± 3.93%, respectively. The various storage conditions; at room temperature or 4°C, with or without light-protection, for 6 months had little effects on the amounts of eluted cefazolin. The results showed both in-housed cefazolin-PMMA beads and cefazolin-calcium sulfate beads could be the effective tools for the treatment of local bacterial infections.
抗生素珠已成为治疗局部细菌感染的常用方法。从市售药用抗生素制备抗生素珠是临床上一种便捷的方法。研究了头孢唑林从聚甲基丙烯酸甲酯（PMMA）（SmartSet HV、Depuy I和Cemfix 3）珠和硫酸钙珠中的洗脱特性。将市售头孢唑林制剂以1 g头孢唑林/10 g基质物质的比例掺入PMMA或硫酸钙中制成珠子。在15天内洗脱的头孢唑林浓度高于金黄色葡萄球菌（ATCC 25923）的最低抑菌浓度（MIC）。PMMA珠洗脱的头孢唑林浓度范围为3.6±1.2至4.6±0.4 mg，硫酸钙珠为15.4±1.7 mg。PMMA珠和硫酸钙珠在15天内累积洗脱的头孢唑林分别为34.41±3.93%至38.67±3.04%和95.94±3.93%。在室温或4°C下，有或没有避光保护，储存6个月，对头孢唑林的洗脱量影响不大。结果表明，自制的头孢唑林-PMMA珠和头孢唑林-硫酸钙珠都可能是治疗局部细菌感染的有效工具。
