Campeiro Junior Luiz D, Rahal Sheila Canevese, Souza Marcos A, Osowski Alini, Silva Júnior José I S
Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Brazil.
Department of Veterinary Pathology, Mato Grosso School of Veterinary Medicine, Cuiabá, Brazil.
Front Vet Sci. 2023 Mar 7;10:1027951. doi: 10.3389/fvets.2023.1027951. eCollection 2023.
The utilization of antibiotic-loaded cement spacer in the induced membrane development has been a debate topic in human medicine. To the best of the author's knowledge, these combinations have not yet been evaluated in birds. Therefore, this study assessed induced membrane formation using radiography and histology, in a segmental defect of a chicken radius, with or without the addition of cefazolin. Thirty 18-month-old healthy chickens were divided into two equal groups: G1-bone defect filled with bone cement; G2-bone defect filled with cefazolin powder-loaded bone cement. Radiographic examinations of the left forearm were taken immediately after surgery and at 7, 15, and 21 postoperative days. For the collection of the induced membranes, five chickens in each group were euthanized at 7, 15, and 21 days after surgery. Radiographically, the bone cement was identified as a radiopaque structure occupying the bone defect in both groups. Mild new bone formation in at least one of the fractured extremities of the bone defect was seen only 21 days after surgery in most chickens. Histologically, there was no difference in the mean thickness of the induced membrane between groups at all time points. Multifocal multinucleated cells differed between groups at 7 (G1 > G2) and 21 (G2 > G1) days after surgery. Mononuclear inflammatory infiltrate differed between groups only on day 21 (G1 > G2). Fibrous tissue proliferation did not differ between groups at all evaluation times. Blood vessel density differed only at 21 days postoperatively (G2 < G1). Multifocal areas of cartilage differed between groups at all time points (G1 > G2). In conclusion, cefazolin mixed with bone cement did not affect thickness of the induced membrane, but did result in a negative effect on some histological aspects, such as fewer vessels, less multifocal areas of cartilage, and persistence of inflammation.
抗生素负载骨水泥间隔物在诱导膜形成中的应用一直是人类医学中的一个争论话题。据作者所知，这些组合尚未在禽类中进行评估。因此，本研究利用X射线摄影和组织学方法，评估了在添加或不添加头孢唑林的情况下，鸡桡骨节段性缺损中诱导膜的形成情况。30只18月龄的健康鸡被分为两组，每组15只：G1组为骨缺损处填充骨水泥；G2组为骨缺损处填充含头孢唑林粉末的骨水泥。术后立即以及术后第7、15和21天对左前臂进行X射线检查。为了收集诱导膜，每组在术后第7、15和21天分别对5只鸡实施安乐死。在X射线片上，骨水泥在两组中均表现为占据骨缺损的不透射线结构。大多数鸡仅在术后21天在骨缺损的至少一个骨折端出现轻度新骨形成。组织学检查发现，所有时间点两组诱导膜的平均厚度均无差异。术后第7天（G1>G2）和第21天（G2>G1）两组间多灶性多核细胞存在差异。仅在术后第21天两组间单核炎性浸润存在差异（G1>G2）。在所有评估时间点两组间纤维组织增生无差异。术后仅第21天两组间血管密度存在差异（G2<G1）。所有时间点两组间软骨多灶性区域存在差异（G1>G2）。总之，头孢唑林与骨水泥混合不影响诱导膜的厚度，但对一些组织学方面有负面影响，如血管减少、软骨多灶性区域减少和炎症持续存在。
