Department of International Trade, Tamkang University, New Taipei, Taiwan, ROC.
Aino Health Science Center, Aino University, Tokyo, Japan.
J Med Microbiol. 2012 Feb;61(Pt 2):223-232. doi: 10.1099/jmm.0.037291-0. Epub 2011 Oct 13.
We evaluated 11 variable number tandem repeat (VNTR) markers for the epidemiological investigation of Salmonella enterica serovar Typhi (S. Typhi) infection and compared the results to those obtained by PFGE. PFGE, using one or two restriction enzymes (XbaI and BlnI), was insufficient to differentiate between some isolates that were epidemiologically unlinked. Multilocus variable-number tandem repeat analysis (MLVA)-8, based on analysis of the eight most variable VNTRs, displayed a high level of discrimination when distinguishing between epidemiologically unlinked isolates that could not be discerned by PFGE with two enzymes. An MLVA-8 typing scheme could be implemented as a routine subtyping tool for the epidemiological investigation of S. Typhi infections. Because seven of the 11 VNTRs are highly variable, the VNTR markers may only be useful in determining genetic relationships among very closely related isolates in short-term epidemiological studies and not for discerning S. Typhi clones.
我们评估了 11 个可变数串联重复（VNTR）标记物，用于沙门氏菌血清型 Typhi（S. Typhi）感染的流行病学研究，并将结果与 PFGE 获得的结果进行了比较。使用一种或两种限制酶（XbaI 和 BlnI）的 PFGE 不足以区分一些在流行病学上没有关联的分离株。基于对 8 个最可变 VNTR 分析的多位点可变数串联重复分析（MLVA）-8，当区分不能通过两种酶的 PFGE 区分的流行病学上无关联的分离株时，显示出很高的区分度。MLVA-8 分型方案可以作为 S. Typhi 感染的流行病学研究的常规亚型分析工具。由于 11 个 VNTR 中有 7 个高度可变，VNTR 标记物可能仅用于确定短期流行病学研究中非常密切相关的分离株之间的遗传关系，而不能用于区分 S. Typhi 克隆。
