Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, Turku, Finland.
J Sep Sci. 2011 Nov;34(22):3158-65. doi: 10.1002/jssc.201100569. Epub 2011 Oct 14.
A complex mixture of procyanidin aglycones was isolated by Sephadex LH-20 column chromatography from the silver birch inner bark, which is a polyphenol-rich source of natural antioxidants. Procyanidins were studied by using hydrophilic interaction liquid chromatography combined with high-resolution ESI-TOF-MS. A good chromatographic separation was achieved and procyanidins eluted according to their increasing degree of polymerization. Individual procyanidins were detected from dimers up to the degree of polymerization of 22 by their negative-ion mass spectra. The results showed that hydrophilic interaction liquid chromatography can be successfully applied for the analysis of high-molecular-weight procyanidins with enhanced sensitivity in electrospray mass spectrometry.
从富含多酚的天然抗氧化剂白桦内皮中，通过 Sephadex LH-20 柱层析分离出一种复杂的原花青素苷元混合物。使用亲水相互作用液相色谱法结合高分辨率 ESI-TOF-MS 研究原花青素。实现了良好的色谱分离，原花青素根据其聚合度的增加而洗脱。通过负离子质谱，从二聚体到聚合度为 22 的单体原花青素都被检测到。结果表明，亲水相互作用液相色谱法可成功应用于分析高分子量原花青素，并在电喷雾质谱中提高了灵敏度。
