Department Chemical Biology, Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
Chembiochem. 2011 Nov 25;12(17):2555-9. doi: 10.1002/cbic.201100578. Epub 2011 Oct 13.
Click the switch: By using a photoswitchable click amino acid (PSCaa) a light-induced intramolecular thiol-ene click reaction with a neighboring cysteine under very mild conditions results in an azobenzene bridge. By expanding the genetic code for PSCaa the specific incorporation of photoswitch units into proteins in living cells can result in an exciting approach for studying light-controllable activity, in vivo.
通过使用光可切换点击氨基酸（PSCaa），在非常温和的条件下，与相邻半胱氨酸发生光诱导的分子内硫醇-烯点击反应，生成偶氮苯桥。通过扩展 PSCaa 的遗传密码，可以将光开关单元特异性地掺入活细胞中的蛋白质中，从而为研究体内光可控活性提供一种令人兴奋的方法。
