使用偏振敏感光学相干断层扫描技术对早期牙齿脱矿进行临床评估。
Clinical assessment of early tooth demineralization using polarization sensitive optical coherence tomography.
作者信息
Louie Tiffany, Lee Chulsung, Hsu Dennis, Hirasuna Krista, Manesh Saman, Staninec Michal, Darling Cynthia L, Fried Daniel
机构信息
Department of Orofacial Sciences, University of California, San Francisco, San Francisco, California 94143-0758, USA.
出版信息
Lasers Surg Med. 2010 Dec;42(10):738-45. doi: 10.1002/lsm.21013.
INTRODUCTION
The aims of this study were to test the hypothesis that polarization sensitive-optical coherence tomography (PS-OCT) can be used to non-destructively measure and quantify the severity of the early demineralization of enamel on buccal and occlusal surfaces and assess the inhibitory effect of fluoride varnish in vivo.
METHODS
A split mouth design was used to assess the effects of fluoride varnish on inhibiting dissolution on 20 test subjects. Orthodontic bands with a buccal window were cemented on the premolars and small incisions were produced on occlusal surfaces to serve as sites for plaque retention for enhanced demineralization. Bands were removed after 30 days and PS-OCT scans were acquired in vivo of occlusal and buccal areas. Teeth were extracted, sectioned, and analyzed using polarized light microscopy (PLM) and transverse microradiography (TMR) for comparison with the PS-OCT images.
RESULTS
High contrast PS-OCT images were acquired of both occlusal and buccal surfaces in vivo. Both occlusal and buccal surfaces, showed a significant difference (P < 0.05) in the integrated reflectivity (ΔR) between the "sound" and "carious" enamel groups. Although the mineral loss (ΔZ) and ΔR measured using TMR and PS-OCT were less for the fluoride varnish-treated buccal surfaces the difference was not statistically significant (P > 0.05).
CONCLUSIONS
Our results indicate that PS-OCT can non-destructively measure early enamel demineralization on the buccal and occulsal surfaces in vivo.
引言
本研究的目的是验证以下假设，即偏振敏感光学相干断层扫描（PS-OCT）可用于无损测量和量化颊面和咬合面早期牙釉质脱矿的严重程度，并评估含氟涂料在体内的抑制作用。
方法
采用双侧对照设计评估含氟涂料对20名受试者牙齿溶解的抑制效果。在双尖牙上粘结带有颊面窗口的正畸带环，并在咬合面制作小切口作为菌斑滞留部位，以增强脱矿。30天后取下带环，对咬合面和颊面进行体内PS-OCT扫描。拔牙后，将牙齿切片，并用偏光显微镜（PLM）和横向显微放射摄影（TMR）进行分析，以与PS-OCT图像进行比较。
结果
在体内获得了咬合面和颊面的高对比度PS-OCT图像。咬合面和颊面“健康”与“龋坏”牙釉质组的积分反射率（ΔR）均存在显著差异（P < 0.05）。尽管用TMR和PS-OCT测量的含氟涂料处理颊面的矿物质损失（ΔZ）和ΔR较小，但差异无统计学意义（P > 0.05）。
结论
我们的结果表明，PS-OCT可在体内无损测量颊面和咬合面早期牙釉质脱矿情况。
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