Department of Ecology and Genetics/Limnology, Uppsala University, Uppsala, Sweden.
PLoS One. 2011;6(10):e25883. doi: 10.1371/journal.pone.0025883. Epub 2011 Oct 6.
We conducted a metacommunity experiment to investigate the role of dispersal for bacterial community composition (BCC) and function of freshwater bacteria. Bacteria were dispersed from a common source pool into three different lake communities in their natural lake water. The experiment was conducted in dialysis bags to enable a decoupling between a change in the local environment and dispersal. BCC was determined by terminal restriction fragment length polymorphism (tRFLP) of the 16S rRNA gene. We show that the greatest changes in BCC occurred between 10% and 43% of dispersal of standing stock per day. Functioning, measured as growth rate, was also affected by dispersal in all three communities but the qualitative pattern differed between communities, sometimes showing a hump-shaped relationship to dispersal and sometimes decreasing with increasing dispersal. In all waters, functioning was related to BCC. Our results show that dispersal does affect BCC and functioning but that high dispersal rates are needed. Further, the effect of dispersal on BCC and function seem to depend on the quality of the habitat to which bacteria disperse into.
我们进行了一个复合种群实验，以研究扩散对淡水细菌群落组成（BCC）和功能的作用。细菌从一个共同的源池中分散到三个不同的湖泊群落中，它们在自然湖水中。该实验是在透析袋中进行的，以实现局部环境变化和扩散之间的解耦。BCC 通过 16S rRNA 基因的末端限制性片段长度多态性（tRFLP）来确定。我们表明，BCC 最大的变化发生在每天 10%到 43%的存量扩散之间。在所有三个群落中，功能（以增长率衡量）也受到扩散的影响，但群落之间的定性模式不同，有时与扩散呈驼峰形关系，有时随扩散增加而减少。在所有水域中，功能与 BCC 相关。我们的结果表明，扩散确实会影响 BCC 和功能，但需要较高的扩散率。此外，扩散对 BCC 和功能的影响似乎取决于细菌分散到的栖息地的质量。
