Area de Microbiologia, Departamento de Biologia Funcional and IUBA, Facultad de Medicina, Universidad de Oviedo, 33006 Oviedo, Spain.
J Proteome Res. 2011 Dec 2;10(12):5481-92. doi: 10.1021/pr200762y. Epub 2011 Nov 3.
Streptomycetes are bacterial species that undergo a complex developmental cycle that includes programmed cell death (PCD) events and sporulation. They are widely used in biotechnology because they produce most clinically relevant secondary metabolites. Although Streptomyces coelicolor is one of the bacteria encoding the largest number of eukaryotic type kinases, the biological role of protein phosphorylation in this bacterium has not been extensively studied before. In this issue, the variations of the phosphoproteome of S. coelicolor were characterized. Most distinct Ser/Thr/Tyr phosphorylation events were detected during the presporulation and sporulation stages (80%). Most of these phosphorylations were not reported before in Streptomyces, and included sporulation factors, transcriptional regulators, protein kinases and other regulatory proteins. Several of the identified phosphorylated proteins, FtsZ, DivIVA, and FtsH2, were previously demonstrated to be involved in the sporulation process. We thus established for the first time the widespread occurrence and dynamic features of Ser/Thr/Tyr protein phosphorylation in a bacteria species and also revealed a previously unrecognized phosphorylation motif "x(pT)xEx".
链霉菌是一种经历复杂发育周期的细菌，包括程序性细胞死亡（PCD）事件和孢子形成。它们在生物技术中被广泛应用，因为它们产生大多数临床上相关的次生代谢物。尽管天蓝色链霉菌是编码最多真核类型激酶的细菌之一，但在该细菌中蛋白质磷酸化的生物学作用以前尚未得到广泛研究。在本期中，我们对天蓝色链霉菌的磷酸蛋白质组的变化进行了特征描述。在孢子前期和孢子形成期（80%）检测到了大多数独特的 Ser/Thr/Tyr 磷酸化事件。这些磷酸化事件中的大多数以前在链霉菌中没有报道过，包括孢子形成因子、转录调节剂、蛋白激酶和其他调节蛋白。先前已证明鉴定出的一些磷酸化蛋白，如 FtsZ、DivIVA 和 FtsH2，参与了孢子形成过程。因此，我们首次在细菌物种中建立了广泛存在和动态的 Ser/Thr/Tyr 蛋白质磷酸化特征，并揭示了一个以前未被识别的磷酸化基序“x(pT)xEx”。
