State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China.
Nat Prod Res. 2012;26(19):1842-7. doi: 10.1080/14786419.2011.607454. Epub 2011 Oct 14.
Ionic liquid was used as extraction solvents and applied to the extraction of tannins from Galla chinensis in the simultaneous ultrasonic- and microwave-assisted extraction (UMAE) technique. Several parameters of UMAE were optimised, and the results were compared with of the conventional extraction techniques. Under optimal conditions, the content of tannins was 630.2 ± 12.1 mg g⁻¹. Compared with the conventional heat-reflux extraction, maceration extraction, regular ultrasound- and microwave-assisted extraction, the proposed approach exhibited higher efficiency (11.7-22.0% enhanced) and shorter extraction time (from 6 h to 1 min). The tannins were then identified by ultraperformance liquid chromatography tandem mass spectrometry. This study suggests that ionic liquid-based UMAE is an efficient, rapid, simple and green sample preparation technique.
离子液体被用作萃取溶剂，并应用于同时超声和微波辅助萃取（UMAE）技术中从五倍子中提取单宁。优化了 UMAE 的几个参数，并将结果与传统萃取技术进行了比较。在最佳条件下，单宁的含量为 630.2 ± 12.1 mg g⁻¹。与传统的热回流萃取、浸渍萃取、常规超声和微波辅助萃取相比，该方法具有更高的效率（提高了 11.7-22.0%）和更短的萃取时间（从 6 小时缩短至 1 分钟）。然后通过超高效液相色谱串联质谱法对单宁进行了鉴定。本研究表明，基于离子液体的 UMAE 是一种高效、快速、简单和绿色的样品制备技术。
