一种简单快速的方法，可排除患有输入性疟疾的旅行者中高疟原虫血症。

A simple and fast method to exclude high Plasmodium falciparum parasitaemia in travellers with imported malaria.

机构信息

Department of Parasitology, Academic Medical Center, Amsterdam, The Netherlands.

出版信息

Malar J. 2011 Oct 14;10:300. doi: 10.1186/1475-2875-10-300.

DOI:10.1186/1475-2875-10-300

PMID:21999570

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3210116/

Abstract

BACKGROUND

Counts of malaria parasites in peripheral blood are important to assess severity of Plasmodium falciparum malaria. Thin and thick smears are routinely used for this purpose.

METHODS

In this study the Binax NOW Malaria Test, an easy-to-perform rapid diagnostic test, with Histidine Rich Protein-2 (HRP-2) and aldolase as diagnostic markers, was used for semi-quantitative assessment of parasitaemia of P. falciparum.

RESULTS

In 257 patients with imported P. falciparum malaria, reactivity of aldolase increased with higher parasitaemia. In all patients with a parasitaemia above 50,000 asexual parasites/μl (> 1%) co-reactivity of HRP-2 and aldolase was observed. Absence of aldolase reactivity in the presence of HRP-2 was a reliable predictive marker to exclude high (> 1%) parasitaemia in P. falciparum malaria.

CONCLUSIONS

Assessment of HRP-2 and aldolase co-reactivity can be of help in clinical decision making in the acute care setting of returning travellers suspected of having malaria.

摘要

背景

外周血中的疟原虫计数对于评估恶性疟原虫疟疾的严重程度很重要。通常使用薄血涂片和厚血涂片来进行这种评估。

方法

本研究中使用了 Binax NOW 疟疾检测，这是一种易于操作的快速诊断检测方法，以 HRP-2 和醛缩酶作为诊断标志物，用于对恶性疟原虫的疟原虫血症进行半定量评估。

结果

在 257 例输入性恶性疟原虫疟疾患者中，随着寄生虫血症的升高，醛缩酶的反应性增加。在所有寄生虫血症高于 50,000 个无性体/μl (> 1%)的患者中，均观察到 HRP-2 和醛缩酶的共同反应性。在存在 HRP-2 的情况下没有醛缩酶反应性是排除恶性疟原虫疟疾中高 (> 1%)寄生虫血症的可靠预测标志物。