苦竹提取物的抗伪狂犬病病毒活性。

Anti pseudorabies virus activity of kumazasa extract.

作者信息

Iwata Kei, Naito Erika, Yamashita Kazutaka, Kakino Kenichi, Taharaguchi Satoshi, Kimachi Yoshihisa, Hara Motonobu, Takase Kozo

机构信息

Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima, Kagoshima, University, Japan

出版信息

Biocontrol Sci. 2010 Dec;15(4):123-8. doi: 10.4265/bio.15.123.

DOI:10.4265/bio.15.123

PMID:21212504

Abstract

Sasa veitchii or "kumazasa" has been used for the preservation of food, or preventing bacterial activity. However, the antiviral activity of kumazasa is poorly understood. In the present study, the antiviral activity of kumazasa extract (KE) was assessed by the plaque reduction assay for the pseudorabies virus (PRV). KE reduced 99% of the plaque formation of PRV at concentrations of 1.2%, showing that KE inhibited PRV adsorption to cells and IE180 expression. The polysaccharide fraction of KE showed a concentration dependent inhibition of PRV plaque formation. We conclude that KE possesses potent anti PRV activity, and the candidate responsible for the antiviral property was the polysaccharide fraction.

摘要

五月竹（又称“苦竹”）已被用于食品保鲜或抑制细菌活性。然而，苦竹的抗病毒活性却鲜为人知。在本研究中，通过噬斑减少试验评估了苦竹提取物（KE）对伪狂犬病病毒（PRV）的抗病毒活性。KE在浓度为1.2%时可减少99%的PRV噬斑形成，表明KE可抑制PRV对细胞的吸附及IE180表达。KE的多糖组分对PRV噬斑形成呈现浓度依赖性抑制作用。我们得出结论，KE具有强大的抗PRV活性，且负责抗病毒特性的候选成分是多糖组分。