Department of Clinical Biochemistry and Immunology, Statens Serum Institut, Copenhagen, Denmark.
Methods. 2012 Feb;56(2):204-12. doi: 10.1016/j.ymeth.2011.09.025. Epub 2011 Oct 7.
Inflammatory markers may have different effects depending on concentration, location in the body and on which other inflammatory markers that is present. The complexity of the immune system thus demands for multiplex analysis of inflammatory markers to get a broad picture of the immune response. This paper describes our experience with analysis of inflammatory markers with multiplex immunoassays based on Luminex xMAP technology through the last ten years. Analysis of several different sample matrixes like plasma/serum, dried blood spot samples, amniotic and cervical fluid, nasal lavage, spinal fluid, and cell culture are described, including the use of internal assay markers to detect problems with samples or analysis. Pre-analytical considerations and steps in preparation of the in-house multiplex assay to decrease inter-assay variations are discussed.
炎症标志物的作用可能因其浓度、在体内的位置以及存在的其他炎症标志物而有所不同。因此，为了全面了解免疫反应，需要对炎症标志物进行多重分析。本文介绍了我们过去十年中使用基于 Luminex xMAP 技术的多重免疫分析方法分析炎症标志物的经验。描述了对几种不同样本基质（如血浆/血清、干血斑样本、羊水和宫颈液、鼻洗液、脑脊液和细胞培养物）的分析，包括使用内部检测标记物来检测样本或分析中存在的问题。讨论了分析前的注意事项以及制备内部多重分析的步骤，以减少分析内的差异。
