Phong D T, Hien V T T, Thanh T T V, Tang D V
Department of Experimental Taxonomy and Genetic Diversity, Vietnam National Museum of Nature, Vietnam Academy for Science and Technology, Hanoi, Vietnam.
Genet Mol Res. 2011 Oct 6;10(4):2382-93. doi: 10.4238/2011.October.6.3.
Dalbergia oliveri is a leguminous tree of the Fabaceae family. This species is popular and valuable in Vietnam and is currently listed on the Vietnam Red List and on the IUCN Red List as endangered. Two PCR techniques using RAPD and inter-simple sequence repeat (ISSR) markers were used to make a comparative analysis of genetic diversity in this species. Fifty-six polymorphic primers (29 RAPD and 27 ISSR) were used. The RAPD primers produced 63 bands across 35 genotypes, of which 24 were polymorphic. The number of amplified bands varied from one to four, with a size range from 250 to 1400 bp. The percentage polymorphism ranged from 0 to 75. Amplification of genomic DNA of the 35 genotypes, using ISSR analysis, yielded 104 fragments, of which 63 were polymorphic. The number of amplified fragments using ISSR primers ranged from one to nine and varied in size from 250 to 1500 bp. The percentage polymorphism ranged from 0 to 100. ISSR markers were relatively more efficient than RAPDs. The mental test between two Jaccard's similarity matrices gave r ≥0.802, showing good fit correlation between ISSRs and RAPDs. Clustering of isolates remained more or less the same for RAPDs compared to combined RAPD and ISSR data. The similarity coefficient ranged from 0.785 to 1.000, 0.698 to 0.956 and 0.752 to 0.964 with RAPD, ISSR, and the combined RAPD-ISSR dendrogram, respectively.
越南黄花梨是豆科的一种豆科树木。该物种在越南很受欢迎且具有重要价值，目前被列入越南红色名录和世界自然保护联盟红色名录，属于濒危物种。使用两种PCR技术，即随机扩增多态性DNA（RAPD）和简单重复序列区间（ISSR）标记，对该物种的遗传多样性进行了比较分析。使用了56个多态性引物（29个RAPD引物和27个ISSR引物）。RAPD引物在35个基因型中产生了63条带，其中24条是多态性的。扩增带的数量从1条到4条不等，大小范围为250至1400碱基对。多态性百分比范围为0至75。使用ISSR分析对35个基因型的基因组DNA进行扩增，产生了104个片段，其中63个是多态性的。使用ISSR引物扩增的片段数量从1条到9条不等，大小在250至1500碱基对之间变化。多态性百分比范围为0至100。ISSR标记相对比RAPD更有效。两个杰卡德相似性矩阵之间的相关性检验得出r≥0.802，表明ISSR和RAPD之间具有良好的拟合相关性。与RAPD和ISSR联合数据相比，RAPD分析的分离株聚类情况大致相同。RAPD、ISSR和RAPD-ISSR联合树形图的相似系数分别为0.785至1.000、0.698至0.956和0.752至0.964。
