Juchum F S, Leal J B, Santos L M, Almeida M P, Ahnert D, Corrêa R X
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
Genet Mol Res. 2007 Sep 30;6(3):543-53.
Dalbergia nigra (rosewood) is a long-lived leguminous species, which is endemic to the Brazilian Atlantic forest. Because of the high economic value of its wood, this species has been over-explored in recent years. Currently, rosewood is included in the IUCN Red List as vulnerable. We examined the genetic diversity of 87 specimens of D. nigra sampled from a continuous forest in the Veracel Reserve and Brazilwood Ecological Station, Porto Seguro, Bahia state, with random amplified polymorphic DNA markers. Grouping analyses were done using unweighted pair group method with arithmetic averages. Using the 16 most informative primers, 112 markers were obtained; 39% (44 bands) were polymorphic. A genetic similarity matrix was made based on the polymorphic bands. The dispersion graph and dendrogram analyses showed three distinct sub-populations. The degree of polymorphism was high, near that of other populations of similar species; however, it was considered low for the conservation of this species.
黑黄檀（巴西红木）是一种长寿的豆科物种，原产于巴西大西洋森林。由于其木材具有很高的经济价值，该物种近年来遭到过度开发。目前，巴西红木被列入世界自然保护联盟红色名录，属于易危物种。我们使用随机扩增多态性DNA标记，对从巴伊亚州塞古罗港维拉塞尔保护区和巴西苏木生态站的一片连续森林中采集的87份黑黄檀样本的遗传多样性进行了检测。分组分析采用算术平均的非加权配对组方法。使用16条信息量最大的引物，共获得112个标记；其中39%（44条带）为多态性标记。基于多态性条带构建了遗传相似性矩阵。散点图和聚类图分析显示出三个不同的亚种群。多态性程度较高，接近其他类似物种的种群；然而，就该物种的保护而言，其多态性程度被认为较低。
