INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria-Pabellón 2, C1428EHA, Buenos Aires, Argentina.
Bull Environ Contam Toxicol. 2012 Jan;88(1):6-9. doi: 10.1007/s00128-011-0423-8.
The toxic effect of sublethal concentrations (1, 17.5 and 35 mg L(-1)) of pure glyphosate was evaluated on acetylcholinesterase (AChE) activity in the fish species, Cnesterodon decemmaculatus. Acute bioassays (96 h) under laboratory conditions were conducted and homogenates for each specimen corresponding to the anterior, middle and posterior body sections were performed. Fish survival was 100%, even at the highest concentration tested (35 mg L(-1)), in accordance with the low lethal toxicity reported for glyphosate. However, a significant inhibitory effect on AChE activity was recorded even for the lowest herbicide concentration tested (1 mg L(-1)), in the homogenates corresponding to the anterior body section. The inhibition ranged from 23 to 36%. The analytical determination of glyphosate in assay media by ion chromatography, was used to verify its stability. These results indicate that AChE-a neurotoxicity biomarker-in C. decemmaculatus may be affected by exposure to environmentally relevant concentrations of glyphosate.
研究了纯草甘膦亚致死浓度（1、17.5 和 35 mg/L）对鱼类 Cnesterodon decemmaculatus 乙酰胆碱酯酶（AChE）活性的毒性作用。在实验室条件下进行了急性生物测定（96 h），并对每个标本的前、中、后体部分进行了匀浆。即使在测试的最高浓度（35 mg/L）下，鱼类的存活率仍为 100%，这与草甘膦报告的低致死毒性一致。然而，即使在测试的最低除草剂浓度（1 mg/L）下，在前体部分的匀浆中也记录到 AChE 活性的显著抑制作用。抑制范围为 23%至 36%。通过离子色谱法在测定介质中分析测定草甘膦，以验证其稳定性。这些结果表明，C. decemmaculatus 中的 AChE-一种神经毒性生物标志物-可能会受到环境相关浓度草甘膦暴露的影响。
