Ruamthum W, Visetson S, Milne J R, Bullangpoti V
Department of Zoology, Faculty of Science, Kasetsart University, 50 Phahon Yothin Road, Chatuchak, Bangkok 10900, Thailand.
Commun Agric Appl Biol Sci. 2011;76(4):923-30.
This study focused on the effects of a glyphosate-based herbicide on activity of the neuron enzyme, acetylcholinesterase (AchE), in the tadpole stage (stage 35-39) of the East Asian Bullfrog, Hoplobatrachus rugulosus. There were 4 herbicide concentration treatments consisting of glyphosate-based herbicide added at 21, 24, 27 and 30 microl to 1L de-chlorinated water in glass containers (10x15x20 cm). There were 4 replicates per treatment, each replicate using 20 tadpoles. The toxicity results were compared with tadpoles in distilled water as a control treatment. After 24, 48, 72 and 96 hours exposure to glyphosate-based herbicide concentrations, LC50 values of 25.21, 24.66, 24.16 and 23.63 microl/L, respectively, were recorded. AChE activities decreased significantly and markedly with herbicide concentration. Such inhibition of AChE activity by this glyphosate-based herbicide indicates the potential of such herbicides to disrupt ecological communities in water near where the herbicides are applied.
本研究聚焦于一种草甘膦基除草剂对东亚牛蛙（虎纹蛙）蝌蚪期（第35 - 39阶段）神经元酶乙酰胆碱酯酶（AchE）活性的影响。有4种除草剂浓度处理，即在玻璃容器（10×15×20厘米）中，向1升去氯水中分别添加21、24、27和30微升草甘膦基除草剂。每个处理有4个重复，每个重复使用20只蝌蚪。将毒性结果与蒸馏水中的蝌蚪作为对照处理进行比较。在暴露于草甘膦基除草剂浓度24、48、72和96小时后，分别记录到半数致死浓度（LC50）值为25.21、24.66、24.16和23.63微升/升。乙酰胆碱酯酶活性随除草剂浓度显著降低。这种草甘膦基除草剂对乙酰胆碱酯酶活性的抑制表明，此类除草剂有可能破坏其施用地点附近水体中的生态群落。
