用于濒危热带树种 Aspidosperma polyneuron（夹竹桃科）的微卫星标记。

Microsatellite markers for Aspidosperma polyneuron (Apocynaceae), an endangered tropical tree species.

机构信息

Programa de Pós-graduação em Biologia Comparada, Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes 3900, 14040-901 Ribeirão Preto, São Paulo, Brazil.

出版信息

Am J Bot. 2011 Nov;98(11):e300-2. doi: 10.3732/ajb.1100222. Epub 2011 Oct 14.

DOI:10.3732/ajb.1100222

PMID:22003175

Abstract

PREMISE OF THE STUDY

This study was designed to develop and characterize a preliminary set of nuclear microsatellite markers for Aspidosperma polyneuron, an endangered tropical tree species.

METHODS AND RESULTS

Leaf samples were collected from a group of mature A. polyneuron trees in a large area along the Pardo and Mogi-Guaçu river basins in São Paulo State in the southeastern region of Brazil. Twenty-five simple sequence repeat (SSR) markers were developed and 16 of 30 samples had polymorphic loci. The number of alleles per locus ranged from two to 17 (average = 7.06). A high diversity (H(e) = 0.65) and parentage exclusion probability for the first (0.9995) and second (0.9999) parents were measured.

CONCLUSIONS

These 16 polymorphic markers suggest a high potential for genetic diversity, gene flow, and mating system analyses in A. polyneuron.

摘要

研究前提

本研究旨在开发和描述一组用于 Aspidosperma polyneuron 的核微卫星标记，Aspidosperma polyneuron 是一种濒危的热带树种。

方法和结果

从巴西东南部圣保罗州帕多和莫吉瓜苏河流域的一个大面积地区的一组成熟的 A. polyneuron 树上采集了叶片样本。开发了 25 个简单序列重复（SSR）标记，其中 30 个样本中有 16 个具有多态性位点。每个位点的等位基因数范围为 2 到 17（平均值 = 7.06）。测量了每个位点的高多样性（H(e) = 0.65）和亲子排除概率（第一代为 0.9995，第二代为 0.9999）。