Department of Food Science, University of Naples "Federico II", Via Universitá 100, 80055 Portici, Napoli, Italy.
Food Chem Toxicol. 2012 Feb;50(2):280-7. doi: 10.1016/j.fct.2011.09.031. Epub 2011 Oct 8.
Cereals products for direct human consumption are rarely contaminated by moulds, unlike raw materials, which are often infected, either in the field or during storage. In this study, 27 samples of dried pasta characterised by size, packaging and marketing intended for young children consumption were collected and analysed by liquid chromatography (LC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for Deoxynivalenol (DON), Ochratoxin A (OTA) and Aflatoxin B(1) (AFB(1)) determination. The samples that showed the highest amounts of one of the mycotoxins were cooked for 10min, digested with an in vitro gastrointestinal protocol and bioaccessibility values were calculated. Seven of the 27 samples exceeded from 120% to 225% the legal limit of 200μg/kg for DON fixed for processed cereal-based baby foods by an European Regulation; all the collected samples were under the OTA legal limit (0.05μg/kg) fixed by the European Regulation and no sample was contaminated by AFB(1) over the instrumental limit of detection of 0.10μg/kg. The mean value of gastric bioaccessibility verified for the DON resulted of 23.1%, whereas mean duodenal bioaccessibility was 12.1%.
供人类直接食用的谷物产品很少受到霉菌污染，与原材料不同，原材料在田间或储存过程中经常受到感染。在这项研究中，收集了 27 份大小、包装和营销方式各异的干意大利面样本，这些样本旨在供幼儿食用，并用液相色谱（LC）和液相色谱-串联质谱（LC-MS/MS）法对脱氧雪腐镰刀菌烯醇（DON）、赭曲霉毒素 A（OTA）和黄曲霉毒素 B(1)（AFB(1)）进行了分析。对其中一种霉菌毒素含量最高的样本进行了 10 分钟的烹饪，用体外胃肠协议进行了消化，并计算了生物可利用性值。在 27 个样本中，有 7 个样本的 DON 含量超过了 225%，达到了欧盟法规规定的加工谷物类婴儿食品 200μg/kg 的法定限量；所有采集的样本均低于欧盟法规规定的 OTA 法定限量（0.05μg/kg），且没有样本的 AFB(1)含量超过仪器检测限 0.10μg/kg。验证 DON 的胃生物利用度平均值为 23.1%，十二指肠生物利用度平均值为 12.1%。
