笼状葡萄糖-6-磷酸用于调控核糖开关活性的光控。
Caged glucosamine-6-phosphate for the light-control of riboswitch activity.
机构信息
University of Bonn, Life and Medical Sciences, c/o Kekulé-Institute for Organic Chemistry and Biochemistry, Bonn, Germany.
出版信息
Photochem Photobiol Sci. 2012 Mar;11(3):489-92. doi: 10.1039/c1pp05242j. Epub 2011 Oct 17.
PMID:22005771
Abstract
We have synthesized a light-activatable ("caged") derivative of glucosamine-6-phosphate (GlcN6P), which only upon irradiation becomes a cofactor for the glmS riboswitch. This glmS riboswitch maintains its activity when embedded in the 3'-untranslated region of eukaryotic mRNA molecules and caged GlcN6P reduces the amount of translated EGFP upon irradiation with light in vitro.
摘要
我们合成了一种可光激活（“笼蔽”）的葡萄糖胺-6-磷酸（GlcN6P）衍生物，只有在辐照后才成为 glmS 核糖开关的辅因子。这种 glmS 核糖开关在真核生物 mRNA 分子的 3'-非翻译区中保持其活性，并且笼蔽的 GlcN6P 在体外用光辐照时减少了翻译的 EGFP 的量。
Zotero 插件