Laboratory of Environment Biomonitoring, Coastal Ecology Unit, Faculty of Sciences of Bizerta, University of Carthage, 7021 Zarzouna, Tunisia.
Environ Monit Assess. 2012 Oct;184(10):5851-61. doi: 10.1007/s10661-011-2385-z. Epub 2011 Oct 18.
Marine gastropods, exposed to anthropogenic pollution, accumulate high chemical concentrations in their tissues, especially in the digestive glands. An evaluation of the impacts of cadmium (Cd) and permethrin (Perm), coupled with reproductive events (pre-spawning and post-spawning phase) throughout the year, was attempted by measuring catalase (CAT), a biomarker of defence, on Hexaplex trunculus experimentally exposed for 48 or 96 h. Specimens of gastropods were sampled from Bizerta Lagoon (Tunisia). The results show that CAT activity increased in gastropods exposed to the three cadmium concentrations (C1 Cd, 100 μg L(-1); C2 Cd, 200 μg L(-1); and C3 Cd, 300 μg L(-1)) and to the three permethrin doses (C1 Perm, 100 μg L(-1); C2 Perm, 150 μg L(-1); and C3 Perm, 200 μg L(-1)) tested. A decrease in CAT was noted in the digestive gland of the H. trunculus exposed to permethrin at the concentration of 200 μg L(-1) during the pre-spawning and post-spawning phases. H. trunculus in post-spawning was more sensitive to cadmium and permethrin than in the pre-spawning phase. The biochemical responses to pollutants (cadmium and permethrin) represented by CAT may act as a biomarker of exposure in this species.
海洋腹足类动物暴露在人为污染下，会在其组织中积累高浓度的化学物质，尤其是在消化腺中。本研究通过测量防御生物标志物过氧化氢酶（CAT），评估了镉（Cd）和氯菊酯（Perm）对海洋腹足类动物的影响，同时结合了全年的生殖事件（产卵前和产卵后阶段）。实验中，Hexaplex trunculus 腹足类动物暴露于三种镉浓度（C1 Cd，100μg/L；C2 Cd，200μg/L；C3 Cd，300μg/L）和三种氯菊酯剂量（C1 Perm，100μg/L；C2 Perm，150μg/L；C3 Perm，200μg/L）下 48 或 96 小时。腹足类动物标本取自突尼斯比塞大泻湖。结果表明，暴露于三种镉浓度（C1 Cd，100μg/L；C2 Cd，200μg/L；C3 Cd，300μg/L）和三种氯菊酯剂量（C1 Perm，100μg/L；C2 Perm，150μg/L；C3 Perm，200μg/L）下的 CAT 活性在腹足类动物中均增加。在产卵前和产卵后阶段，暴露于 200μg/L 氯菊酯的 H. trunculus 消化腺中 CAT 活性下降。与产卵前阶段相比，产卵后的 H. trunculus 对镉和氯菊酯更敏感。CAT 代表的污染物（镉和氯菊酯）的生化反应可以作为该物种暴露的生物标志物。
